IL-13: Human Interleukin-13

Product Sheet RP1085

Description

BACKGROUND Interleukin-13 (IL-13) is an important cytokine secreted from Th2 cells. The functions attributed to IL-13 overlap significantly with those of IL-4 (induces IgE secretion from B cells and inhibits expression of inflammatory cytokines such as IL-1β, TNF-α, IL-8 and IL-6), but differs from IL-4 in that IL-13 seems to link inflammatory response of immune cells to the pathophysiological changes in the surrounding non-immune cells. The receptor subunits of IL-13 consist of IL-4Rα, IL-13Rα1 and IL-13Rα2. Human and mouse IL-13 are cross-reactive.

Recombinant human IL-13 is a non-glycosylated protein, containing 114 amino acids and having a molecular mass of 12.5 kDa.

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat. No.:
RP1085
 
Alternative Name:
NC30
 
Source:
E. coli
 
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
 
Formulation:
Recombinant human IL-13 is lyophilized from 10 mM Sodium Citrate, pH 3.0.
 
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
 
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
 
Biological Activity:
The activity is determined by the dose dependent proliferation of TF-1 cells and is typically less than 1 ng/mL.
 
AA Sequence:
SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCPHKVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GRFN
 

Products

Product Size CAT.# Price Quantity
Human Interleukin-13 (IL-13): Human Interleukin-13 Size: 10 ug CAT.#: RP1085-10 Price: $194.00
-+
Human Interleukin-13 (IL-13): Human Interleukin-13 Size: 100 ug CAT.#: RP1085-100 Price: $624.00
-+
Human Interleukin-13 (IL-13): Human Interleukin-13 Size: 1000 ug CAT.#: RP1085-1000 Price: $5,327.00
-+

Resources/Documents

Product Sheet RP1085