Product Sheet CL0782

Description

BACKGROUND The IL-1 family of cytokines is produced by activated monocytes/macrophages during the acute phase of inflammatory response. The IL-1 cytokines are also produced by a variety of other cells including neutrophiles, keratinocytes, epithelial cells, fibroblasts, adrenal cortical and medullary cells.1 The cytokine IL-1 and IL-1 have a 25% homology in amino acids. The biological action of IL-1 cytokines is the results of their binding to the IL-1 receptor type I (Rt1). A second IL-1 receptor (Rt2) does not produce any biological effect and most probably represents a “decoy receptor”. A third member of IL-1 family of cytokines is IL-1 receptor antagonist (IL-1 Ra), which exhibits same binding affinity to both types of IL-1 receptors, but without inducing a biological response, thus antagonizing the effects of IL-1.2 Interestingly, the action of IL-1 Ra is not restricted to the counterregulation of inflammatory processes. IL-1 Ra has been shown to be upregulated in the serum of obese subjects.3
 
REFERENCES  
1. Carter, D. B. et al: Nature. 344:633, 1990.
2. Granowitz, E. V. et al: J. Biol. Chem. 266:14147, 1991.
3. Saltevo, J. et al: Diab Vasc Dis Res. 4:328, 2007.
 
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

IL-1RA Standard Curve: Using the Human IL-1RA ELISA Kit, O.D. data was graphed against IL-1RA protein concentration. The TMB reaction was incubated at 37°C for 15 min.

Details

Cat.No.:
CL0782
Target Protein Species:
Human
Range:
31.2pg/ml-2000pg/ml
Specificity:
No detectable cross-reactivity
with any other cytokine.
Storage:
Store at 4°C. Use within 6 months.
 
ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.

Products

Product Size CAT.# Price Quantity
Human IL-1RA ELISA Kit: Human Interleukin Receptor-1 Antagonist ELISA Kit Size: 96 Wells CAT.#: CL0782 Price: $581.00
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