IL-21: Human Interleukin-21

Product Sheet RP1090
Product Sheet RP1090AF

Description

BACKGROUND Interleukin-21 (IL-21) is a member of the common gamma-chain family of cytokines (includes IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15) and is expressed by activated CD4+ T cells. IL-21 has a private receptor known as IL-21R, which is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple Ab classes in vivo. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells.

Recombinant human IL-21 is a non-glycosylated protein, containing 133 amino acids and having a total molecular mass of 15.5 kDa.

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat. No.:
RP1090 / RP1090AF
 
Alternative Name:
None
 
Source:
E. coli
 
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
 
Formulation:
Recombinant human IL-21 is lyopohilized from 20 mM sodium phosphophate buffer, pH 7.5.
 
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
 
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
 
Biological Activity:
The activity iss determined by the dose-dependent stimulation of Mino cells and is typcially less than 0.1-0.6 ng/mL.
 
AA Sequence:
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
 
Animal Component-Free
This product is produced with no animal derived raw products.  All processing and handling employs animal free equipment and animal free protocols.
 

Products

Product Size CAT.# Price Quantity
Human Interleukin-21 (IL-21): Human Interleukin-21 Size: 10 ug CAT.#: RP1090-10 Price: $194.00
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Human Interleukin-21 (IL-21): Human Interleukin-21 Size: 100 ug CAT.#: RP1090-100 Price: $624.00
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Human Interleukin-21 (IL-21): Human Interleukin-21 Size: 1000 ug CAT.#: RP1090-1000 Price: $5,327.00
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Human IL-21, Animal-Free: Human Interleukin-21, Animal-Free Size: 10 ug CAT.#: RP1090AF-10 Price: $213.00
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Human IL-21, Animal-Free: Human Interleukin-21, Animal-Free Size: 100 ug CAT.#: RP1090AF-100 Price: $687.00
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Human IL-21, Animal-Free: Human Interleukin-21, Animal-Free Size: 1000 ug CAT.#: RP1090AF-1000 Price: $5,860.00
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Resources/Documents

Product Sheet RP1090
Product Sheet RP1090
Product Sheet RP1090AF