5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Brochure Cell Applications Inc
Instructions MCoN
Description
MCoN (Mouse Cortical Neurons) are cryopreserved primary mouse neurons derived from the cerebral cortex of day 18 embryonic CD1 mouse brains. When cultured under the recommended conditions, MCoN arborize and form complex neurite network in one week. MCoN Stain positive for β III-Tubulin.
The cerebral cortex plays a key role in cognition, memory, attention, perceptual awareness, thought, language and consciousness. Neurons connect to other neural cells through neurites and synapses to form complex neurocircuitry for signal transmission. A healthy circuitry in the brain is essential for cognitive functions, proper control and regulation of the other parts of the body. Dysfunction and degeneration in the cerebral cortex have been implicated in various brain injuries, neurological and psychiatric disorders. Cortical neurons, therefore, provide an excellent cellular model system to study disease mechanisms and pathophysiologies. They also serve as a platform for drug screening and development, toxicity tests, immunostaining, live cell imaging, co-culturing and electrophysiology.
(Click to Enlarge) Freshly isolated Mouse Cortical Neurons (MCoN) cultured for 7 days. A: Phase contrast, 100x. B: Immunostaining for β-III Tubulin (green), GFAP (red), DAPI (blue), 100x.
Details
| Tissue | Normal healthy mouse brain |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for β-III Tubulin |
| Bioassay | Plate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med |
| Cryovial | 2M MCoN cryopreserved after isolation in Serum-Free Freezing Medium (042-50) |
| Kit | 2M cryopreserved MCoN, Coating Soln I (027-05), Plating Med (M817P-10), Culture Med (M817-100) |
| Doublings | N/A - Neurons don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Mouse Cortical Neurons Total Kit: 2x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: M882NK-20 | Price: $685.00 | |
| Cryopreserved Mouse Cortical Neurons (MCoN): Frozen MCoN (2x10^6). | Size: 1 Cryovial | CAT.#: M882N-20 | Price: $555.00 | |
| Cryopreserved MCoN Total Kit: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: M882NK-10 | Price: $540.00 | |
| Cryopreserved MCoN: Frozen MCoN (1x10^6) | Size: 1 Cryovial | CAT.#: M882N-10 | Price: $418.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Neuron Cell Growth Medium, 100ml: All-in-one ready-to-use media | Size: 100 ml | CAT.#: M817-100 | Price: $90.00 | |
| Mouse Neuron Culture Medium Kit, 100ml: Basal Medium and Growth Supplement sold together, bottled separatedly | Size: Yields 100 ml | CAT.#: M817K-100 | Price: $99.00 | |
| Mouse Neuron Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 100 ml | CAT.#: M816-100 | Price: $40.00 | |
| Mouse Neuron Culture Supplement: Added to Basal Medium to create Culture Medium | Size: 3 ml | CAT.#: M817-GS | Price: $64.00 | |
| Mouse Neuron Plating Solution, 20ml: For initial plating of neurons | Size: 20 ml | CAT.#: M817P-20 | Price: $44.00 | |
| Mouse Neuron Plating Solution, 10ml: For initial plating of neurons | Size: 10 ml | CAT.#: M817P-10 | Price: $23.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 10 ml | CAT.#: 027-10 | Price: $36.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 5 ml | CAT.#: 027-05 | Price: $18.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $465.00 | |
| Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $62.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 |
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