Instructions MMcg
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Brochure Cell Applications Inc
Description
Mouse Microglia (MMcg) are primary mouse microglia derived from day 1 postnatal CD1 mouse brains. When revived and plated and cultured under recommended conditions, MMcg form adherent culture.
Microglia, originated from the hematopoietic stem cells in bone marrow, are the resident macrophages in the central nervous system (CNS) which is separated from the rest of the body due to the presence of blood-brain barrier. Microglia actively survey the surrounding area and respond by scavenging damaged neural cells, plaques, and infectious agents. Due to their functions in immune response and maintaining homeostasis in the CNS, microglia have been implicated in neurodevelopment, CNS plasticity and repair, neuroinflammation, aging and neurodegeneration, neuropathic pain, and infections.
Characterization: MMcg stain positive for F4/80, and uptake DiI-Ac-LDL
A: MMcg culture on day 4, 100x. B: Immunostaining in MMcg culture on day 4 for F4/80 (red) and DAPI (blue), 200x. C: DiI-Ac-LDL uptake (red) in MMcg culture on day 4, 100x.
Details
| Tissue | Normal, healthy mouse brain |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for F4/80 |
| Bioassay | Plate, spread on CC ware & uptake DiI-Ac-LDL |
| Cryovial | 1M MMcg frozen in Microglia Cell Freezing Med (043-50) |
| Kit | Cryovial Frozen MMcg (M8816K-10n), Microglia Cltr Med Kit (M619K-100) |
| Doublings | N/A |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Mouse Microglia Total Kit: 1x10^6 Cells (Neonatal) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: M8816K-10n | Price: $795.00 | |
| Cryopreserved Mouse Microglia (MMcg), neonatal: Frozen MMcg (1x10^6) | Size: 1 Cryovial | CAT.#: M8816-10n | Price: $720.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Macrophage Culture Medium Kit: Basal medium & culture supplement sold together packaged separately | Size: Yields 100 ml | CAT.#: M619K-100 | Price: $85.00 | |
| Freezing Medium for Macrophages & Microglia Cells: Specialized freezing medium with higher FBS concentration, more suitable for Macrophages & Microglia Cells. | Size: 50 ml | CAT.#: 043-50 | Price: $85.00 |
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