Instructions MNSC
MSDS Cryopreserved Cells
Cell Apps Flyer Brain Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
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Description

Mouse Neural Stem Cells (MNSC) are multipotent, and can be induced to differentiate into neurons, astrocytes and oligodendrocytes that make up the central nervous system (CNS). Neurospheres transplanted into intact brain can survive, expand and differentiate into mature neurons, astrocytes and oligodendrocytes in 3 weeks. The ability of neural stem cells to retain multi-lineage potential and proliferate extensively in vitro provides new avenues for the treatment of neural degenerative diseases and injuries.
Isolated from fetal or adult mouse brains, MNSC are maintained in an undifferentiated, proliferative state by culturing them as free floating neurospheres in serum-free medium optimized with growth factors. MNSC are cryopreserved at first passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation. Differentiated MNSCs are positive for β-tubulin III, GFAP and the oligodendrocyte marker O4 when cultured in Mouse Neural Differentiation Medium.  The cells form neurospheres in Mat Neural Stem Cell Growth Medium.

(Click to Enlarge) Mouse Neural Stem Cells (MNSC) (A) and Differentiated MNSC immunofluorescently stained with antibodies to βIII-tubulin (B), GFAP (C) and oligodendrocyte marker O4 (D).

Details

Tissue
Normal healthy mouse brain cerebral cortex
QC
No bacteria, yeast, fungi, mycoplasma
Character
β-tubulin III, GFAP & O4 (+) in Diff Med
Bioassay
Form neurospheres in MNSC Gr Med
Cryovial
2,000,000 MNSC (1st psg) cryopreserved in Freezing Med (040-50)
Kit
Frozen cryovial MNSC (MS820-20f), Grwth Med (R813-250), Neural Stem Cell Dissociation Solution (076-20), 10cm non-TC dish x2
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates) 
Doublings
At least 2
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions MNSC

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Products

Product Size CAT.# Price Quantity
Cryopreserved MNSC, Fetal: 2x10^6 Cells (Fetal), Medium & Culture Dishes Cells (See Details tab for specifics) Size: 1 Kit CAT.#: MS820K-20f Price: $853.00
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Cryopreserved MNSC, Fetal: Frozen MNSC (2x10^6) Size: 1 Cryovial CAT.#: MS820-20f Price: $709.00
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Proliferating MNSC, Fetal: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: MS821-25f Price: $709.00
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Proliferating MNSC, Fetal: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: MS821-75f Price: $914.00
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Proliferating MNSC, Fetal: Actively growing, dividing cells, in medium Size: 24 Well CAT.#: MS821-24Wf Price: $914.00
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Proliferating MNSC, Fetal: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: MS821-96Wf Price: $1,043.00
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Related Products

Product Size CAT.# Price Quantity
MNSC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 250 ml CAT.#: M812-250 Price: $40.00
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MNSC Differentiation Medium: General MNSC Differentiation Medium Size: 250 ml CAT.#: M813D-250 Price: $128.00
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MNSC Differentiation Medium, Astrocyte Lineage: Differentiates MNSC to Astrocytes Size: 100 ml CAT.#: M813D-100A Price: $62.00
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MNSC Differentiation Medium, Neuron Lineage: Differentiates MNSC to Neurons Size: 100 ml CAT.#: M813D-100N Price: $74.00
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MNSC Differentiation Medium, Oligodendrocyte Lineage: Differentiates MNSC to Oligodendrocytes Size: 100 ml CAT.#: M813D-100O Price: $62.00
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MNSC Growth Medium: All-in-one ready-to-use Size: 250 ml CAT.#: M813-250 Price: $118.00
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MNSC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 250 ml CAT.#: M813K-250 Price: $128.00
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MNSC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 10 ml CAT.#: M813-GS Price: $88.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Resources/Documents

Cell Apps Flyer Brain Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now