Instructions HPBT
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human Peripheral Blood T Cells (HPBT) provide a useful in vitro tool for studying the biology and pathology of CD4+ cells. HPBT cells play critical roles in the regulation of immune responses, and are responsible for mediating many of the effector mechanisms of the immune system. Increased activity in the field of immunotherapy, coupled with the expense of clinical trials, has led to renewed interest in obtaining high purity human T cells to meet needs for research and development. Our CD4+ T Cells are isolated from human blood of healthy donors and cryopreserved immediately after isolation.
Details
Tissue | Normal healthy human peripheral blood |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | positive selection directly against CD4 |
Cryovial | 5,000,000 HPBT frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO |
Kit | Cryovial Frozen HPBT (6902-50a), Maintenance Medium (615-250) |
Doublings | N/A - Blood cells don't proliferate in vitro |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Cryopreserved Human Peripheral Blood CD4+ T Cells Total Kit, adult: 5x10^6 Cells CD4+ T Cells (Adult) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: 6902K-50a | Price: $891.00 | |
Cryopreserved Peripheral Blood T Cells (HPBT), adult: Frozen HPBT, CD4+ | Size: 1 Cryovial | CAT.#: 6902-50a | Price: $825.00 |
Related Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Human Blood Cell Culture Medium: Optimized and ready for use. | Size: 250 ml | CAT.#: 615-250 | Price: $76.00 | |
Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately | Size: Yields 250 ml | CAT.#: 615K-250 | Price: $87.00 | |
Human Blood Cell Basal Medium: Basal medium (contains no growth supplement). Add Supplement before use. | Size: 250 ml | CAT.#: 614-250 | Price: $49.00 | |
Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium | Size: 40 ml | CAT.#: 615-CS | Price: $52.00 |
Extended Family Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Polyclonal C-C Chemokine Receptor 5 Rabbit Antibody: Polyclonal C-C Chemokine Receptor 5 Rabbit Antibody | Size: 100 ul | CAT.#: CA0707 | Price: $302.00 | |
Rabbit CCR7 Antibody: Rabbit CCR7 Antibody | Size: 100 ul | CAT.#: CG1678 | Price: $384.00 | |
Mouse Cluster of Differentiation 4 Antibody: Mouse Cluster of Differentiation 4 Antibody | Size: 100 ul | CAT.#: CP10035 | Price: $302.00 | |
Rabbit CD40 Antibody: Rabbit CD40 Antibody | Size: 100 ul | CAT.#: CA1019 | Price: $302.00 | |
Mouse Cluster of Differentiation 40 Antibody: Mouse Cluster of Differentiation 40 Antibody | Size: 100 ul | CAT.#: CP10045 | Price: $333.00 | |
Polyclonal CD40 Ligand Antibody: Polyclonal CD40 Ligand Antibody | Size: 100 ul | CAT.#: CA1020 | Price: $302.00 | |
Mouse Cluster of Differentiation 45 Antibody: Mouse Cluster of Differentiation 45 Antibody | Size: 100 ul | CAT.#: CP10047 | Price: $302.00 | |
Mouse Interleukin-2 Antibody: Mouse Interleukin-2 Antibody | Size: 100 ul | CAT.#: CP10324 | Price: $302.00 | |
Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | Size: 100 ul | CAT.#: CP10325 | Price: $302.00 | |
Anti-LFA-1a: Rabbit LFA-1a/CD11a/ITGAL Antibody | Size: 100 ul | CAT.#: CG1754 | Price: $295.00 | |
Rabbit Tumor Necrosis Factor Receptor 1 Antibody: Rabbit Tumor Necrosis Factor Receptor 1 Antibody | Size: 100 ul | CAT.#: CG1602 | Price: $384.00 | |
Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells | Size: 50 ml | CAT.#: 040-50BC | Price: $75.00 | |
Human CD40 Ligand (CD40L): Human Cluster of Differentiation 40 Ligand | Size: 10 ug | CAT.#: RP1025-10 | Price: $86.00 | |
Human CD40 Ligand (CD40L): Human Cluster of Differentiation 40 Ligand | Size: 100 ug | CAT.#: RP1025-100 | Price: $317.00 | |
Human CD40 Ligand (CD40L): Human Cluster of Differentiation 40 Ligand | Size: 1000 ug | CAT.#: RP1025-1000 | Price: $1,561.00 | |
Human CD40/TNFRSF5 ELISA Kit: Human Cluster of Differentiation 40/Tumor necrosis factor ligand superfamily member 5 ELISA Kit | Size: 96 Wells | CAT.#: CL0702 | Price: $511.00 | |
Human CD40L ELISA Kit: Human Cluster of Differentiation 40 Ligand ELISA Kit | Size: 96 Wells | CAT.#: CL0573 | Price: $533.00 | |
Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
Human Interferon-Gamma (IFN-gamma): Human Interferon-gamma | Size: 20 ug | CAT.#: RP1077-20 | Price: $86.00 | |
Human Interferon-Gamma (IFN-gamma): Human Interferon-gamma | Size: 100 ug | CAT.#: RP1077-100 | Price: $194.00 | |
Human Interferon-Gamma (IFN-gamma): Human Interferon-gamma | Size: 1000 ug | CAT.#: RP1077-1000 | Price: $1,184.00 | |
Human IFN gamma ELISA Kit: Human Interferon gamma ELISA Kit | Size: 96 Wells | CAT.#: CL0373 | Price: $554.00 | |
Human Interleukin-2 (IL-2): Human Interleukin-2 | Size: 10 ug | CAT.#: RP1012-10 | Price: $86.00 | |
Human Interleukin-2 (IL-2): Human Interleukin-2 | Size: 100 ug | CAT.#: RP1012-100 | Price: $317.00 | |
Human Interleukin-2 (IL-2): Human Interleukin-2 | Size: 1000 ug | CAT.#: RP1012-1000 | Price: $839.00 | |
Human IL-2 ELISA Kit: Human Interleukin-2 ELISA Kit | Size: 96 Wells | CAT.#: CL0397 | Price: $484.00 | |
Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 10 ug | CAT.#: RP1021-10 | Price: $194.00 | |
Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 100 ug | CAT.#: RP1021-100 | Price: $484.00 | |
Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 1000 ug | CAT.#: RP1021-1000 | Price: $4,090.00 | |
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor | Size: 20 ug | CAT.#: RP1008-20 | Price: $194.00 | |
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor | Size: 100 ug | CAT.#: RP1008-100 | Price: $484.00 | |
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor | Size: 1000 ug | CAT.#: RP1008-1000 | Price: $3,444.00 | |
Human Interferon Inducible T cell Alpha Chemokine (I-TAC / CXCL11): Human Interferon-Inducible T cell alpha Chemokine | Size: 20 ug | CAT.#: RP1128-20 | Price: $194.00 | |
Human Interferon Inducible T cell Alpha Chemokine (I-TAC / CXCL11): Human Interferon-Inducible T cell alpha Chemokine | Size: 100 ug | CAT.#: RP1128-100 | Price: $484.00 | |
Human Interferon Inducible T cell Alpha Chemokine (I-TAC / CXCL11): Human Interferon-inducible T cell alpha Chemokine | Size: 1000 ug | CAT.#: RP1128-1000 | Price: $3,175.00 | |
Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 20 ug | CAT.#: RP1010-20 | Price: $194.00 | |
Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 100 ug | CAT.#: RP1010-100 | Price: $484.00 | |
Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 1000 ug | CAT.#: RP1010-1000 | Price: $3,175.00 | |
Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit | Size: 96 Wells | CAT.#: CL0410 | Price: $517.00 | |
Human L-Selectin ELISA Kit: Human L-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0503 | Price: $500.00 | |
Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha | Size: 20 ug | CAT.#: RP1147-20 | Price: $194.00 | |
Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha | Size: 100 ug | CAT.#: RP1147-100 | Price: $484.00 | |
Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha | Size: 1000 ug | CAT.#: RP1147-1000 | Price: $3,175.00 | |
Human MIP-3a ELISA Kit: Human Macrophage Inflammatory Protein-3 alpha ELISA Kit | Size: 96 Wells | CAT.#: CL0453 | Price: $495.00 | |
Human Regulated upon Activation, Normal T Cell Expressed & Secreted (RANTES): Human Regulated on Activation Normal T Cell Expressed & Secreted | Size: 20 ug | CAT.#: RP1138-20 | Price: $194.00 | |
Human Regulated upon Activation, Normal T Cell Expressed & Secreted (RANTES): Human Regulated on Activation Normal T Cell Expressed & Secreted | Size: 100 ug | CAT.#: RP1138-100 | Price: $484.00 | |
Human Regulated upon Activation, Normal T Cell Expressed & Secreted (RANTES): Human Regulated on Activation Normal T Cell Expressed & Secreted | Size: 1000 ug | CAT.#: RP1138-1000 | Price: $3,175.00 | |
Human Rantes ELISA Kit: Human Regulated Upon Activation, Normal T Cell Expressed and Secreted ELISA Kit | Size: 96 Wells | CAT.#: CL0494 | Price: $500.00 | |
Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Factor-alpha | Size: 10 ug | CAT.#: RP1111-10 | Price: $78.00 | |
Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Factor-alpha | Size: 1000 ug | CAT.#: RP1111-1000 | Price: $3,175.00 | |
Human TNF-alpha ELISA Kit: Human Tumor Necrosis Factor alpha ELISA Kit | Size: 96 Wells | CAT.#: CL0525 | Price: $517.00 | |
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free | Size: 20 ug | CAT.#: RP1008AF-20 | Price: $213.00 | |
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free | Size: 100 ug | CAT.#: RP1008AF-100 | Price: $533.00 | |
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1008AF-1000 | Price: $3,788.00 | |
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 20 ug | CAT.#: RP1010AF-20 | Price: $213.00 | |
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 100 ug | CAT.#: RP1010AF-100 | Price: $533.00 | |
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 1000 ug | CAT.#: RP1010AF-1000 | Price: $3,492.00 | |
Human IL-2, Animal-Free: Human Interleukin-2, Animal-Free | Size: 10 ug | CAT.#: RP1012AF-10 | Price: $95.00 | |
Human IL-2, Animal-Free: Human Interleukin-2, Animal-Free | Size: 100 ug | CAT.#: RP1012AF-100 | Price: $347.00 | |
Human IL-2, Animal-Free: Human Interleukin-2, Animal-Free | Size: 1000 ug | CAT.#: RP1012AF-1000 | Price: $923.00 | |
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 10 ug | CAT.#: RP1021AF-10 | Price: $213.00 | |
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 100 ug | CAT.#: RP1021AF-100 | Price: $533.00 | |
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 1000 ug | CAT.#: RP1021AF-1000 | Price: $4,499.00 | |
Human CD40L, Animal-Free: Human Cluster of Differentiation 40 Ligand, Animal-Free | Size: 10 ug | CAT.#: RP1025AF-10 | Price: $95.00 | |
Human CD40L, Animal-Free: Human Cluster of Differentiation 40 Ligand, Animal-Free | Size: 100 ug | CAT.#: RP1025AF-100 | Price: $347.00 | |
Human CD40L, Animal-Free: Human Cluster of Differentiation 40 Ligand, Animal-Free | Size: 1000 ug | CAT.#: RP1025AF-1000 | Price: $1,717.00 | |
Human IFN-g, Animal-Free: Human Interferon-gamma, Animal-Free | Size: 20 ug | CAT.#: RP1077AF-20 | Price: $95.00 | |
Human IFN-g, Animal-Free: Human Interferon-gamma, Animal-Free | Size: 100 ug | CAT.#: RP1077AF-100 | Price: $213.00 | |
Human IFN-g, Animal-Free: Human Interferon-gamma, Animal-Free | Size: 1000 ug | CAT.#: RP1077AF-1000 | Price: $1,302.00 | |
Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Facto-alpha | Size: 100 ug | CAT.#: RP1111-100 | Price: $317.00 | |
Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free | Size: 10 ug | CAT.#: RP1111AF-10 | Price: $85.00 | |
Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free | Size: 100 ug | CAT.#: RP1111AF-100 | Price: $347.00 | |
Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free | Size: 1000 ug | CAT.#: RP1111AF-1000 | Price: $3,492.00 |
Resources/Documents
Citations
Publications
2016
Nishimura, M., T. Wada, C. Shirakawa, and M. Doi. 2016. Trehalose and Dextran-Containing Solution for Transplanting Mammalian Cells. Patent Application US 20160120170 A1.
Teramura, Y., S. Asif, K. Ekdahl, E. Gustafson and B. Nilsson. 2016. Cell adhesion induced using surface modification with cell-penetrating peptide-conjugated PEG-lipid: A new cell glue for 3D cell-based structures. ACS Appl Mater Interfaces, DOI: 10.1021/acsami.6b14584.