MSDS Cryopreserved Cells
Instructions BPASMC
Cell Apps Flyer Smooth Muscle Cells
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Cell Apps Flyer Airway Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
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Description
Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC) from Cell Applications, Inc. have been used in studies relevant to
- Pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells, the process shown to be mediated by hypoxia-induced expression of Bcl-xl
- Additionally, they were used to elucidate the PKC-α dependent mechanisms of proMMP-2 activation and other signaling events in smooth muscle cells under pro-inflammatory conditions related to cell growth
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Bovine Pulmonary Artery Smooth Muscle Cells, BPASMC (L) immunolabeled for smooth muscle-specific alpha-actin using ABC method (R).
Details
Tissue | Normal healthy bovine pulmonary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 BPASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen BPASMC (B352-05), Growth Medium (B311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | Can be cultured at least 16 doublings |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Bovine Pulmonary Artery Smooth Muscle Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: B352K-05 | Price: $889.00 | |
| Cryopreserved Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Frozen BPASMC (5x10^5) | Size: 1 Cryovial | CAT.#: B352-05 | Price: $715.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: B353-25 | Price: $715.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: B353-75 | Price: $905.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: B353-24W | Price: $905.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: B353-96W | Price: $1,025.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Bovine SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: B310-500 | Price: $74.00 | |
| Bovine SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: B311-500 | Price: $121.00 | |
| Bovine SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: B311K-500 | Price: $130.00 | |
| Bovine SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml (2 parts) | CAT.#: B311-GS | Price: $74.00 | |
| Bovine SMC Induction Medium: Provides signals that change cell behavior | Size: 250 ml | CAT.#: B311I-250 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2016
Chowdhury, A., J. Sarkar, P. Pramanik, T. Chakraborti, S. and S. Chakraborti. 2016. Cross talk between MMP2-Spm-Cer-S1P and ERK1/2 in proliferation of pulmonary artery smooth muscle cells under angiotensin II stimulation. Archives of Biochemistry and Biophysics, 603:91–101.
Chowdhury, A., S. Nandy, J. Sarkar, T. Chakraborti and S. Chakraborti. 2011. Inhibition of pro-/active MMP-2 by green tea catechins and prediction of their interaction by molecular docking studies. Molec Cell Biochem, DOI 10.1007/s11010-016-2903-y.
Sarkar, J., S. Nandy, A. Chowdhury, T. Chakraborti and S. Chakraborti. 2016. Inhibition of MMP-9 by green tea catechins and prediction of their interaction by molecular docking analysis. Biomed & Pharmacother, 84:340-347.
Sarkar, J., A. Chowdhury, T. Chakraborti and S. Chakraborti. 2016. Cross-talk between NADPH oxidase-PKCα-p38MAPK and NF-κB-MT1MMP in activating proMMP-2 by ET-1 in pulmonary artery smooth muscle cells. Molec & Cell Biochm, 415:13-28.
2015
Chakraborti, S., A. Chowdhury and T. Chakraborti. 2015. Cross-talk between p38MAPK and Giα in regulating cPLA2 activity by ET-1 in pulmonary smooth muscle cells. Mol Cell Biochem, 400: 107.
Chowdhury, A., J. Sarkar, T. Chakraborti and S. Chakraborti. 2015. Role of Spm–Cer-S1P signalling pathway in MMP-2 mediated U46619-induced proliferation of pulmonary artery smooth muscle cells: protective role of epigallocatechin-3-gallate. Cell Biochem & Function, 33:463-477.
Rahaman, S., K. Dey, T. Chakraborti and S. Chakraborti. 2015. Angiotensin II inhibits Na+/K+ATPase activity in pulmonary artery smooth muscle cells via glutathionylation and with the involvement of a 15.6 kDa inhibitor protein. Indian J Biochem & Biophys, 52:119-124.
2014
Chowdhury, A., S. Roy, T. Chakraborti, K. Dey and S. Chakraborti. 2014. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molec & Cell Biochem, 385:53-68.
Rahaman, S., K. Dey, P. Das, S. Roy, T. Chakraborti and S. Chakraborti. 2014. Identification, purification and partial characterization of low molecular weight protein inhibitor of Na+/K+-ATPase from pulmonary artery smooth muscle cells. Molec & Cellular Biol, 393:309-317.
2013
Chowdhury, A., S. Roy, T. Chakraborti, K. Dey, and S. Chakraborti. 2013. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molecular and cellular biochemistry:1-16.
Roy, S., T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2013. Role of PKC-α in NF-κB–MT1-MMP–mediated activation of proMMP-2 by TNF-α in pulmonary artery smooth muscle cells. Journal of biochemistry. 153:289-302.
Shaikh, S., J. Sarkar, A. Pramanik, K. Karmakar, and S. Chakraborti. 2013. Effect of m-calpain in PKCα-mediated proliferation of pulmonary artery smooth muscle cells by low dose of ouabain. IJBB. 50:419-427.
2012
Dey, K., S. Roy, B. Ghosh, and S. Chakraborti. 2012. Role of protein kinase C in phospholemman mediated regulation of α2β1 isozyme of Na+K+ ATPase in caveolae of pulmonary artery smooth muscle cells. Biochimie. 94:991-1000.
2011
Roy, S., K. Samanta, T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2011. Role of TGF-β1 and TNF-α in IL-1β mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: Involvement of an aprotinin sensitive protease. Archives of biochemistry and biophysics. 513:61-69.
2009
Chakraborti, S., A. Chowdhury, P. Kar, P. Das, S. Shaikh, S. Roy, and T. Chakraborti. 2009. Role of protein kinase C in NADPH oxidase derived O2(*-)-mediated regulation of Kv–LVOCC axis under U46619 induced increase in [Ca2+] in pulmonary smooth muscle cells. Archives of biochemistry and biophysics. 487:123-130.
2007
Suzuki, Y.J., H. Nagase, C.M. Wong, S.V. Kumar, V. Jain, A.-M. Park, and R.M. Day. 2007. Regulation of Bcl-xL expression in lung vascular smooth muscle. American journal of respiratory cell and molecular biology. 36:678.