MSDS Cryopreserved Cells
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5 Important Cell Culture Rules
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Description
Bovine Endometrial Epithelial Cells (BEnEpC) from Cell Applications, Inc. provide an excellent model system to study many aspects of epithelial function and disease, especially those related to the endometrium and pregnancy-related signaling, such as seminal plasma induced prostaglandins F2α, (PGF2α), E2 (PGE2) and interleukin-6 release by endometrial cells, or the role of PLA in mediating oxytocin-stimulated production of prostaglandins. BEnEpC have also been used to demonstrate the association between variants of gap junctions related genes and conception rate, and to investigate cellular adherence and cytotoxicity of Histophilus somni.
(Click to Enlarge) Bovine Endometrial Epithelial Cells: BEnEpC. Isolated from the inner membrane of the mammalian uterus.
Details
Tissue | Normal healthy endometrial epithelium |
QC | No bacteria, yeast, fungi, mycoplasma |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovail | 500,000 BEnEpC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen BEnEpC (B932-05), Growth Medium (B911-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 10 |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Bovine Endometrium Epithelial Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: B932K-05 | Price: $900.00 | |
| Cryopreserved Bovine Endometrial Epithelial Cells (BEnEpC): Frozen BEnEpC (5x10^5) | Size: 1 Cryovial | CAT.#: B932-05 | Price: $726.00 | |
| Proliferating Bovine Endometrial Epithelial Cells (BEnEpC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: B933-25 | Price: $726.00 | |
| Proliferating Bovine Endometrial Epithelial Cells (BEnEpC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: B933-75 | Price: $916.00 | |
| Proliferating Bovine Endometrial Epithelial Cells (BEnEpC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: B933-24W | Price: $916.00 | |
| Proliferating Bovine Endometrial Epithelial Cells (BEnEpC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: B933-96W | Price: $1,036.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Bovine Endometrial Cell Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: B910-500 | Price: $74.00 | |
| Bovine Endometrial Cell Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: B911-500 | Price: $121.00 | |
| Bovine Endometrial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: B911K-500 | Price: $130.00 | |
| Bovine Endometrial Cell Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml x 2 | CAT.#: B911-GS | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $465.00 | |
| Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2016
Brockus, K., C. Hart, C. Gilfeather, B. Fleming, and C. Lemley. 2016. Dietary melatonin alters uterine artery hemodynamics in pregnant Holstein heifers. Domestic Animal Endocrinology, 55:1-10.
Gilfeather, C. and C. Lemley. 2016. Effects of Interferon-Tau and Steroids on Cytochrome P450 Activity in Bovine Endometrial Epithelial Cells. Reproduction in Domestic Animals, 51:415-420.
Sugimoto, M., Y. Gotoh, T. Kawahara and Y. Sugimoto. 2016. Family with Sequence Similarity 213, Member A is Associated with the Fertility Selection Index in Holsteins. J Fertility Miomarkers, Vol-1 Issue 1 Pg. no.- 20.
2013
Madej, M., C. Hansen, A. Johannisson, and A. Madej. 2013. Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells. Science Research (Natural Science), 5:21-30.
Malgorzata, M., H. Claus, J. Anders, and M. Andrzej. 2013. Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells. Natural Science. 5:21.
Sugimoto, M., S. Sasaki, Y. Gotoh, Y. Nakamura, Y. Aoyagi, T. Kawahara, and Y. Sugimoto. 2013. Genetic variants related to gap junctions and hormone secretion influence conception rates in cows. Proceedings of the National Academy of Sciences. 10.1073/pnas.1309307110
2012
Madej, M., M. Norrby, M.T. Madsen, A. Johannisson, C. Hansen, and A. Madej. 2012. The Effect of Boar Seminal Plasma on the Release of Prostaglandins and Interleukin-6 by Porcine Endometrial and Cervical Cells and Bovine Endometrial Cells. Reproduction in Domestic Animals. 47:113-124.
2010
Norrby, M. 2010. Hormones and sex steroid receptors in the female pig. Vol. 2010. Swedish University of Agricultural Sciences, PhD dissertation.
2009
Hoshinoo, K., K. Sasaki, A. Tanaka, L.B. Corbeil, and Y. Tagawa. 2009. Virulence attributes of Histophilus somni with a deletion mutation in the ibpA gene. Microbial Pathogenesis. 46:273-282.
Krishnaswamy, N., G. Danyod, P. Chapdelaine, and M.A. Fortier. 2009. Oxytocin Receptor Down-Regulation Is Not Necessary for Reducing Oxytocin-Induced Prostaglandin F2α Accumulation by Interferon-τ in a Bovine Endometrial Epithelial Cell Line. Endocrinology. 150:897-905.
2007
Tithof, P.K., M.P. Roberts, W. Guan, M. Elgayyar, and J.D. Godkin. 2007. Distinct phospholipase A2 enzymes regulate prostaglandin E2 and F2alpha production by bovine endometrial epithelial cells. Reproductive Biology and Endocrinology. 5:16.