Instructions REK
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description
Rat Epidermal Keratinocytes (REK) from Cell Applications, Inc. provide a good model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology.
- It was shown that TGFβ1 treatment decreased c-Myc mRNA in REK which, taken together with the TGFβ1-induced growth arrest exhibited by keratinocytes and opposite results obtained in other cells, suggests a correlation between c-Myc mRNA expression and the mitogenic response.
- Additionally, REK were used to demonstrate that pathological increases in keratinocyte sodium channels Nav1.1, Nav1.6, and Nav1.8 expression may contribute to pain by increasing epidermal ATP release, resulting in excessive activation of P2X receptors on primary sensory axons.
- REK were also used to demonstrate that laminin 332 deposition is inhibited by ionizing radiation and, in combination with slower keratinocyte migration, can contribute to the delayed wound healing of irradiated skin.
(Click to Enlarge) Rat Epidermal Keratinocytes: REK. Keratin producing cells isolated from skin tissue.
Details
Tissue | Normal healthy rat skin |
QC | No bacteria, yeast, fungi, mycoplasma |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 REK (primary culture) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen REK (R102-05n), Growth Medium (R131-250) |
Proliferating | Shipped in Gr Med, 1st psg (flasks or plates) |
Doublings | REK do not expand and cannot be subcutured. Thaw & plate REK directly to assay format. REK will undergo only 1 or 2 doublings. |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Epidermal Keratinocyte Total Kit, neonatal: 5x10^5 Cells (Neonatal) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: R102K-05n | Price: $497.00 | |
| Cryopreserved Rat Epidermal Keratinocytes (REK), neonatal: Frozen REK (5x10^5) | Size: 1 Cryovial | CAT.#: R102-05n | Price: $425.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), neonatal: Actively growing, dividing cells, in medium | Size: T-25 Flask | CAT.#: R103-25n | Price: $425.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), neonatal: Actively growing, dividing cells, in medium | Size: T-75 Flask | CAT.#: R103-75n | Price: $615.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), neonatal: Actively growing, dividing cells, in medium | Size: 24 Well | CAT.#: R103-24Wn | Price: $615.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), neonatal: Actively growing, dividing cells, in medium | Size: 96 Well | CAT.#: R103-96Wn | Price: $735.00 | |
| Cryopreserved Rat Epidermal Keratinocyte Total Kit, adult: 5x10^5 Cells (Adult) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: R102K-05a | Price: $519.00 | |
| Cryopreserved Rat Epidermal Keratinocytes (REK), adult: Frozen REK (5x10^5) | Size: 1 Cryovial | CAT.#: R102-05a | Price: $447.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), adult: Actively growing, dividing cells, in medium | Size: T-25 Flask | CAT.#: R103-25a | Price: $447.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), adult: Actively growing, dividing cells, in medium | Size: T-75 Flask | CAT.#: R103-75a | Price: $637.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), adult: Actively growing, dividing cells, in medium | Size: 24 Well | CAT.#: R103-24Wa | Price: $637.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), adult: Actively growing, dividing cells, in medium | Size: 96 Well | CAT.#: R103-96Wa | Price: $757.00 | |
| Cryopreserved Rat Epidermal Keratinocyte Total Kit, fetal: 5x10^5 Cells (Fetal) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: R102K-05f | Price: $497.00 | |
| Cryopreserved Rat Epidermal Keratinocytes (REK), fetal: Frozen REK (5x10^5) | Size: 1 Cryovial | CAT.#: R102-05f | Price: $425.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), fetal: Actively growing, dividing cells, in medium | Size: T-25 Flask | CAT.#: R103-25f | Price: $425.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), fetal: Actively growing, dividing cells, in medium | Size: T-75 Flask | CAT.#: R103-75f | Price: $615.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), fetal: Actively growing, dividing cells, in medium | Size: 24 Well | CAT.#: R103-24Wf | Price: $615.00 | |
| Proliferating Rat Epidermal Keratinocytes (REK), fetal: Actively growing, dividing cells, in medium | Size: 96 Well | CAT.#: R103-96Wf | Price: $735.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| REK Basal Medium: Basal Medium without Growth Supplement | Size: 250 ml | CAT.#: R130-250 | Price: $59.00 | |
| REK Growth Medium: All-in-one ready-to-use media | Size: 250 ml | CAT.#: R131-250 | Price: $82.00 | |
| REK Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 250 ml | CAT.#: R131K-250 | Price: $92.00 | |
| REK Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 1 ml (2 parts) | CAT.#: R131-GS | Price: $44.00 |
Resources/Documents
Citations
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2011
Jourdan, M., A. Lopez, E. Olasz, N. Duncan, M. Demara, W. Kittipongdaja, B. Fish, M. Mäder, A. Schock, and N. Morrow. 2011. Laminin 332 deposition is diminished in irradiated skin in an animal model of combined radiation and wound skin injury. Radiation research. 176:636-648.
2009
Jasinski, K. 2009. Impact of Gaseous Nitric Oxide and Carbon Monoxide on Normal Excisional, Diabetic Excisional and Burn Wound Healing. PhD Dissertation, Ohio State University.
2008
Nakai, T., J. Mochida, and D. Sakai. 2008. Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGF beta-1 in cultured rat nucleus pulposus cells. Arthritis research & therapy. 10:R140.Zhao, P., T.P. Barr, Q. Hou, S.D. Dib-Hajj, J.A. Black, P.J. Albrecht, K. Petersen, E. Eisenberg, J.P. Wymer, and F.L. Rice. 2008. Voltage-gated sodium channel expression in rat and human epidermal keratinocytes: evidence for a role in pain. Pain. 139:90-105.
Zhao, P., T.P. Barr, Q. Hou, S.D. Dib-Hajj, J.A. Black, P.J. Albrecht, K. Petersen, E. Eisenberg, J.P. Wymer, and F.L. Rice. 2008. Voltage-gated sodium channel expression in rat and human epidermal keratinocytes: evidence for a role in pain. Pain. 139:90-105.