Instructions REK
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skin Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Epidermal Keratinocytes (REK) from Cell Applications, Inc. provide a good model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology.  
 
  • It was shown that TGFβ1 treatment decreased c-Myc mRNA in REK which, taken together with the TGFβ1-induced growth arrest exhibited by keratinocytes and opposite results obtained in other cells, suggests a correlation between c-Myc mRNA expression and the mitogenic response. 
  • Additionally, REK were used to demonstrate that pathological increases in keratinocyte sodium channels Nav1.1, Nav1.6, and Nav1.8 expression may contribute to pain by increasing epidermal ATP release, resulting in excessive activation of P2X receptors on primary sensory axons. 
  • REK were also used to demonstrate that laminin 332 deposition is inhibited by ionizing radiation and, in combination with slower keratinocyte migration, can contribute to the delayed wound healing of irradiated skin.
(Click to Enlarge) Rat Epidermal Keratinocytes: REK.  Keratin producing cells isolated from skin tissue.

Details

Tissue
Normal healthy rat skin
QC
No bacteria, yeast, fungi, mycoplasma
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 REK (primary culture) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit 
Cryovial frozen REK (R102-05n), Growth Medium (R131-250)
Proliferating
Shipped in Gr Med, 1st psg (flasks or plates)
Doublings
REK do not expand and cannot be subcutured. Thaw & plate REK directly to assay format. REK will undergo only 1 or 2 doublings.
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions REK

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved REK, Adult: Frozen REK (5x10^5) Size: 1 Cryovial CAT.#: R102-05a Price: $447.00
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Cryopreserved REK, Adult: 5x10^5 Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: R102K-05a Price: $508.00
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Cryopreserved REK, Neonatal: 5x10^5 Cells (Neonatal) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: R102K-05n Price: $486.00
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Cryopreserved REK, Neonatal: Frozen REK (5x10^5) Size: 1 Cryovial CAT.#: R102-05n Price: $425.00
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Proliferating REK, Adult: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: R103-25a Price: $425.00
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Proliferating REK, Adult: Actively growing, dividing cells, in medium Size: 24 Well CAT.#: R103-24Wa Price: $630.00
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Proliferating REK, Adult: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: R103-75a Price: $630.00
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Proliferating REK, Adult: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: R103-96Wa Price: $759.00
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Proliferating REK, Neonatal: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: R103-25n Price: $507.00
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Proliferating REK, Neonatal: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: R103-75n Price: $662.00
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Proliferating REK, Neonatal: Actively growing, dividing cells, in medium Size: 24 Well CAT.#: R103-24Wn Price: $662.00
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Proliferating REK, Neonatal: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: R103-96Wn Price: $764.00
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Related Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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REK Basal Medium: Basal Medium without Growth Supplement Size: 250 ml CAT.#: R130-250 Price: $45.00
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REK Growth Medium: All-in-one ready-to-use media Size: 250 ml CAT.#: R131-250 Price: $71.00
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REK Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 250 ml CAT.#: R131K-250 Price: $80.00
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REK Growth Supplement: Added to Basal Medium to create Growth Medium Size: 1 ml (2 parts) CAT.#: R131-GS Price: $34.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Skin Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2011
Jourdan, M., A. Lopez, E. Olasz, N. Duncan, M. Demara, W. Kittipongdaja, B. Fish, M. Mäder, A. Schock, and N. Morrow. 2011. Laminin 332 deposition is diminished in irradiated skin in an animal model of combined radiation and wound skin injury. Radiation research. 176:636-648.
2009
Jasinski, K. 2009. Impact of Gaseous Nitric Oxide and Carbon Monoxide on Normal Excisional, Diabetic Excisional and Burn Wound Healing. PhD Dissertation, Ohio State University.
2008
Nakai, T., J. Mochida, and D. Sakai. 2008. Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGF beta-1 in cultured rat nucleus pulposus cells. Arthritis research & therapy. 10:R140.Zhao, P., T.P. Barr, Q. Hou, S.D. Dib-Hajj, J.A. Black, P.J. Albrecht, K. Petersen, E. Eisenberg, J.P. Wymer, and F.L. Rice. 2008. Voltage-gated sodium channel expression in rat and human epidermal keratinocytes: evidence for a role in pain. Pain. 139:90-105.
Zhao, P., T.P. Barr, Q. Hou, S.D. Dib-Hajj, J.A. Black, P.J. Albrecht, K. Petersen, E. Eisenberg, J.P. Wymer, and F.L. Rice. 2008. Voltage-gated sodium channel expression in rat and human epidermal keratinocytes: evidence for a role in pain. Pain. 139:90-105.