Product Sheet RP1018
Product Sheet RP1018AF
Description
BACKGROUND Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF.
Recombinant human PDGF-BB is a non-glycosylated homodimer. It is comprised of two, disulfide-linked, 109 amino acid proteins and has a total molecular mass of 24.3 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP1018 / RP1018AF
Alternative Name:
GDGF, ODGF
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant human PDGF-BB is lyophilized from 10 mM Acetic Acid.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is calculated by the dose-dependent proliferation of BALB/c 3T3 indicator cells and is typically 1-3 ng/mL.
AA Sequence:
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KPIFKKATVT LGDHLACKCE TVAAARPVT
Animal Component-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Platelet Derived Growth Factor-BB (PDGF-BB): Human Platelet Derived Growth Factor-BB | Size: 10 ug | CAT.#: RP1018-10 | Price: $194.00 | |
| Human Platelet Derived Growth Factor-BB (PDGF-BB): Human Platelet Derived Growth Factor-BB | Size: 100 ug | CAT.#: RP1018-100 | Price: $484.00 | |
| Human Platelet Derived Growth Factor-BB (PDGF-BB): Human Platelet Derived Growth Factor-BB | Size: 1000 ug | CAT.#: RP1018-1000 | Price: $4,090.00 | |
| Human PDGF-BB, Animal-Free: Human Platelet Derived Growth Factor-BB, Animal-Free | Size: 10 ug | CAT.#: RP1018AF-10 | Price: $213.00 | |
| Human PDGF-BB, Animal-Free: Human Platelet Derived Growth Factor-BB, Animal-Free | Size: 100 ug | CAT.#: RP1018AF-100 | Price: $533.00 | |
| Human PDGF-BB, Animal-Free: Human Platelet Derived Growth Factor-BB, Animal-Free | Size: 1000 ug | CAT.#: RP1018AF-1000 | Price: $4,499.00 |