MSDS Cryopreserved Cells
Instructions PAOEC
5 Important Cell Culture Rules
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Porcine Aortic Endothelial Cells (PAOEC) provide a useful model system to study many aspects of cardiovascular function and disease.  Co-culture of the artery endothelial cells with species-matched smooth muscle cells provides an ideal model for studying the interaction between these two cell types.
 
PAOEC from Cell Applications, Inc., and they have been utilized in a number of research publications, including those demonstrating that:
  • H2O2 contributes to vascular dysfunction
  • Cell survival under oxidative stress depends on phosphorylation of VEGFR-3 
  • Replication of swine fever virus regulates signal transduction pathways and gene expression
  • Direct interactions between endothelial cells and T cells trigger release of proinflammatory molecules that play a role in graft rejection
  • The tetraspanin CD82 is the recognition sensor responsible for rejection of xenotransplants
  • Endothelial cells from heart valves align perpendicular to flow, while aortic endothelial cells align parallel to flow, indicating the need to match the cell types when designing engineered tissue devices
  • Shear stress induces changes in expression of NO synthase, VCAM-1, c-jun, MCP-1 and ICAM-1
  • Swine-to-human xenotransplantation can be improved by pretreating donors with vasopressin
  • Endothelial implants can be designed to increase lumen diameter and replace heart valves
(Click to Enlarge) Porcine Aortic Endothelial Cells: PAOEC

Details

Tissue
Normal healthy porcine aorta
QC
No bacteria, yeast, fungi, mycoplasma
Character
DiI-Ac-LDL uptake: Positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 PAOEC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen PAOEC, Growth Medium (P211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions PAOEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved PAOEC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: P304K-05 Price: $780.00
Cryopreserved PAOEC: Frozen PAOEC (5x10^5) Size: 1 Cryovial CAT.#: P304-05 Price: $630.00
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Proliferating PAOEC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: P305-25 Price: $630.00
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Proliferating PAOEC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: P305-75 Price: $834.00
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Proliferating PAOEC: Actively growing, dividing cells in medium Size: 24 Well CAT.#: P305-24W Price: $834.00
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Proliferating PAOEC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: P305-96W Price: $963.00
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Related Products

Product Size CAT.# Price Quantity
Porcine EC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: P210-500 Price: $54.00
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Porcine EC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: P211-500 Price: $105.00
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Porcine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: P211K-500 Price: $113.00
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Porcine EC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: P211-GS Price: $57.00
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Extended Family Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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PAOEC RNA : Total RNA prepared from Porcine Aortic Endothelial Cells Size: 10 ug CAT.#: P304-R10 Price: $350.00
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PAOEC RNA : Total RNA prepared from Porcine Aortic Endothelial Cells Size: 25 ug CAT.#: P304-R25 Price: $700.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017
Bidarimath, M., K. Khalaj, R. Kridli, F. Kan, M. Koti, and C. Tayade. 2017. Extracellular vesicle mediated intercellular communication at the porcine maternal-fetal interface: A new paradigm for conceptus-endometrial cross-talk. Scientific Reports, doi:10.1038/srep40476.
2016
Ameer, G., M. Kibbe and A. Webb. 2016. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20160199535 A1.
Bidarimath, M. 2016. Immune-Angiogenesis Mechanisms Associated with Porcine Pregnancy Success and Failure. PhD Thesis, Queen’s University.
Dushpanova, A., S. Agostini, E. Ciofini, M. Cabiati, V. Casieri, M. Matteucci, S. Del Ray, A. Clerico, S. Berti and V. Lionetti. 2016. Gene silencing of endothelial von Willebrand Factor attenuates angiotensin II-induced endothelin-1 expression in porcine aortic endothelial cells. Scientific Reports, 6:30048.
Grossini, E., S. Farruggio, F. Qoqaiche, G. Raina, L. Camillo, L. sigaudo, D. Mary, N. Surico and D. Surico. 2016. Monomeric adiponectin modulates nitric oxide release and calcium movements in porcine aortic endothelial cells in normal/high glucose conditions. Life Sciences, 161:1-9.
Roshan, A., L. Dong, C. Alvan, J. Bart and H. Wenhu. 2016. Validation of Cross-Species Reactivity of the VEGF-A/PDGFRβ Bifunctional Antibody PF-06653157J Ocular Pharmacol & Ther, doi:10.1089/jop.2016.0027.
Roshan, A., L. Dong, C. Alvan, J. Bart and H. Wenhu. Validation of Cross-Species Reactivity of the VEGF-A/PDGFRβ Bifunctional Antibody PF-06653157J Ocular Pharmacol & Ther, 32:650-658.
2015
Grossini, E., P. Marotta, S. Farruggio, L. Sigaudo, F. Qoqaiche, G. Raina, V. Giuli, D. Mary, G. VAcca, and F. Pollastro. 2015. Effects of Artemetin on Nitric Oxide Release and Protection against Peroxidative Injuries in Porcine Coronary Artery Endothelial Cells. Phytotherapy Research, doi: 10.1002/ptr.5386.
Nimlamool, W., R. Andrews and M. Falk. 2015. Connexin43 phosphorylation by PKC and MAPK signals VEGF-mediated gap junction internalization. Mol Biol Cell, 26:2755-2768.
Nugent, H. and E. Edelman. Methods and compositions for enhancing vascular access. Patent US 9023377 B2.
Viator, R., H. Khader, N. Hingorani, S. Long, V. Solodushko and B. Fouty. 2015. Hypoxia-induced increases in glucose uptake do not cause oxidative injury or advanced glycation end-product (AGE) formation in vascular endothelial cells.  Physiological Reports, 3:e12460.
2014
Sierad, L., E. Shaw, R. Launius, S. McBride, C. Storholt, R. Poole, D. Spence, K. Miller, L. Sosdian, K. Allen, L. Burton, A. Iari, J. Nagatomi, and D. Simionesuc. 2014. Toward an Endothelial-Cell Covered Mechanical Valve; Surface Re-Engineering and Bioreactor Testing of Mechanical Heart Valves. Challenges in Regenerative Medicine, 1: Aug.
2013
Saleh, S.M., R.S. Parhar, R.S. Al-Hejailan, R.H. Bakheet, H.S. Khaleel, H.G. Khalak, A.S. Halees, M.Z. Zaidi, B.F. Meyer, and G.P. Yung. 2013. Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation. J.  of Immunology. 191:2796-2805.
Saleh, S.M., R.S. Parhar, R.S. Al-Hejailan, R.H. Bakheet, H.S. Khaleel, H.G. Khalak, A.S. Halees, M.Z. Zaidi, B.F. Meyer, G.P. Yung, J.D. Seebach, W. Conca, K.S. Khabar, K.S. Collison, and F.A. Al-Mohanna. 2013. Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation. The Journal of Immunology. 191:2796-2805.
2011
Wang, C.-Y., H.-I. Yeh, T.-J. Chang, H.-J. Hsiao, M.-S. Tsai, S.-M. Tsai, and P.-A. Liu. 2011. Attenuation of nitric oxide bioavailability in porcine aortic endothelial cells by classical swine fever virus. Archives of virology. 156:1151-1160.
2010
Hsiao, H.-J., P.-A. Liu, H.-I. Yeh, and C.-Y. Wang. 2010. Classical swine fever virus down-regulates endothelial connexin 43 gap junctions. Archives of virology. 155:1107-1116.
Sierad, L., A. Simionescu, C. Albers, J. Chen, J. Maivelett, M. Tedder, J. Liao, and D. Simionescu. 2010. Design and Testing of a Pulsatile Conditioning System for Dynamic Endothelialization of Polyphenol-Stabilized Tissue Engineered Heart Valves. Cardiovascular Engineering and Technology. 1:138-153.
2009
Ameer, G., M. Kibbe, and A. Webb. 2009. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20100036476 A1.
2008
Allen, J., S. Khan, M.C. Serrano, and G. Ameer. 2008. Characterization of porcine circulating progenitor cells: toward a functional endothelium. Tissue Engineering Part A. 14:183-194.
Kim, Y.T., H.J. Lee, S.W. Lee, J.Y. Kim, H.C. Wi, S.J. Park, S.J. Bae, and H.J. Kang. 2008. Pre-treatment of porcine pulmonary xenograft with desmopressin: a novel strategy to attenuate platelet activation and systemic intravascular coagulation in an ex-vivo model of swine-to-human pulmonary xenotransplantation. Xenotransplantation. 15:27-35.
Meyer, R.D., D.B. Sacks, and N. Rahimi. 2008. IQGAP1-dependent signaling pathway regulates endothelial cell proliferation and angiogenesis. PloS one. 3:e3848.
Witting, P.K., B.S. Rayner, B.-J. Wu, N.A. Ellis, and R. Stocker. 2008. Hydrogen peroxide promotes endothelial dysfunction by stimulating multiple sources of superoxide anion radical production and decreasing nitric oxide bioavailability. Cellular Physiology and Biochemistry. 20:255-268.
2007
Kawahara, K., K. Setoyama, K. Kikuchi, K. Biswas, R. Kamimura, M. Iwata, T. Ito, Y. Morimoto, T. Hashiguchi, S. Takao, and I. Maruyama. 2007. HMGB1 release in co-cultures of porcine endothelial and human T cells. Xenotransplantation, 14:636-641.
2006
Zhang, W.-Y., E. Schwartz, Y. Wang, J. Attrep, Z. Li, and P. Reaven. 2006. Elevated Concentrations of Nonesterified Fatty Acids Increase Monocyte Expression of CD11b and Adhesion to Endothelial Cells. Arteriosclerosis, Thrombosis, and Vascular Biology. 26:514-519.
2004
Wang, J., X. Zhang, and J. Groopman. 2004. Activation of Vascular Endothelial Growth Factor Receptor-3 and Its Downstream Signaling Promote Cell Survival under Oxidative Stress. J Biol Chem 279:27088-27097.
2002
Butcher, J. and R. Nerem. 2002. Morphological differences between aortic and aortic valve endothelial cells in static and fluid flow conditions. Engineering in Medicine and Biology, Biomedical Engineering Society EMBS/BMES Conference. 318-319, DOI10.1109/IEMBS.2002.1134512.