MSDS Cryopreserved Cells
Instructions PAOSMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Porcine Aortic Smooth Muscle Cells (PAOSMC) from Cell Applications, Inc. were used to investigate
  • Effects of heparin on smooth muscle cell proliferation
  • Heparin-mediated induction of MKP-1 phosphatase, which dephosphorylates ERK, decreasing its activity
  • Normalization of myocardin signaling in the diseased heart
  • Restoration of heart function by targeted shRNA inhibition of myocardin genes
Finally, PAOSMC along with Porcine Aortic Endothelial cells, both from Cell Applications, Inc., have been used as controls in a study characterizing circulating endothelial progenitor cells
Characterization: positive for smooth muscle cell specific alpha-actin expression

Porcine Aortic Smooth Muscle Cells: PAOSMC (L), immunolabeled for smooth muscle-specific alpha-actin using the ABC method (R).

Details

Tissue
Normal healthy porcine aorta
QC
No bacteria, yeast, fungi, mycoplasma
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryopreserved
500,000 PAOSMC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen PAOSMC (P354-05), Grwth Med (P311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, psg 2 (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions PAOSMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved PAOSMC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: P354K-05 Price: $769.00
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Cryopreserved PAOSMC: Frozen PAOSMC (5x10^5) Size: 1 Cryovial CAT.#: P354-05 Price: $619.00
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Proliferating PAOSMC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: P355-25 Price: $619.00
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Proliferating PAOSMC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: P355-75 Price: $823.00
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Proliferating PAOSMC: Actively growing, dividing cells in medium Size: 24 Well CAT.#: P355-24W Price: $823.00
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Proliferating PAOSMC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: P355-96W Price: $952.00
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Related Products

Product Size CAT.# Price Quantity
Porcine SMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: P310-500 Price: $57.00
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Porcine SMC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: P311-500 Price: $105.00
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Porcine SMC Growth Supplement: Added to Basal Growth Medium to create Growth Medium Size: 50 ml CAT.#: P311-GS Price: $57.00
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Porcine SMC Induction Medium: Provides signals that change cell behavior Size: 250 ml CAT.#: P311I-250 Price: $62.00
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Extended Family Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2016
Ameer, G., M. Kibbe and A. Webb. 2016. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20160199535 A1.
Pastrana, H., C. Cooper, M. Alucozai, L. Reece and A. Avila. 2016. Synthesis and in vitro safety assessment of magnetic bacterial cellulose with porcine aortic smooth muscle cells. J Biomed Mat Res, 104:2801-2801.
2014
Gilotti, A. W. Nimlamool, R. Pugh, J. Slee, T. Barthol, E. Miller, and L. Lowe-Krentz. 2014. Heparin Responses in Vascular Smooth Muscle Cells Involve cGMPDependent Protein Kinase (PKG). Journal of Cellular Physiology 229: 2142–2152.
2011
Torrado, M., R. Iglesias, A. Centeno, E. López, and A.T. Mikhailov. 2011. Targeted gene-silencing reveals the functional significance of myocardin signaling in the failing heart. PloS one. 6:e26392.
2010
Blaukovitch, C.I., R. Pugh, A.C. Gilotti, D. Kanyi, and L.J. LoweKrentz. 2010. Heparin treatment of vascular smooth muscle cells results in the synthesis of the dualspecificity phosphatase MKP1. J. cellular biochemistry. 110:382-391.
2009
Ameer, G., M. Kibbe, and A. Webb. 2009. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20100036476 A1.
2008
Allen, J., S. Khan, M.C. Serrano, and G. Ameer. 2008. Characterization of porcine circulating progenitor cells: toward a functional endothelium. Tissue Engineering Part A. 14:183-194.