MSDS Cryopreserved Cells
Instructions PAOSMC
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Description
Porcine Aortic Smooth Muscle Cells (PAOSMC) from Cell Applications, Inc. were used to investigate
- Effects of heparin on smooth muscle cell proliferation
- Heparin-mediated induction of MKP-1 phosphatase, which dephosphorylates ERK, decreasing its activity
- Normalization of myocardin signaling in the diseased heart
- Restoration of heart function by targeted shRNA inhibition of myocardin genes
Finally, PAOSMC along with Porcine Aortic Endothelial cells, both from Cell Applications, Inc., have been used as controls in a study characterizing circulating endothelial progenitor cells
Characterization: positive for smooth muscle cell specific alpha-actin expression
Porcine Aortic Smooth Muscle Cells: PAOSMC (L), immunolabeled for smooth muscle-specific alpha-actin using the ABC method (R).
Details
Tissue | Normal healthy porcine aorta |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryopreserved | 500,000 PAOSMC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen PAOSMC (P354-05), Grwth Med (P311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, psg 2 (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Porcine Aortic Smooth Muscle Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: P354K-05 | Price: $949.00 | |
| Cryopreserved Porcine Aortic Smooth Muscle Cells (PAOSMC): Frozen PAOSMC (5x10^5) | Size: 1 Cryovial | CAT.#: P354-05 | Price: $775.00 | |
| Proliferating Porcine Aortic Smooth Muscle Cells (PAOSMC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: P355-25 | Price: $775.00 | |
| Proliferating Porcine Aortic Smooth Muscle Cells (PAOSMC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: P355-75 | Price: $965.00 | |
| Proliferating Porcine Aortic Smooth Muscle Cells (PAOSMC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: P355-24W | Price: $965.00 | |
| Proliferating Porcine Aortic Smooth Muscle Cells (PAOSMC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: P355-96W | Price: $1,085.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Porcine SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: P310-500 | Price: $74.00 | |
| Porcine SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: P311-500 | Price: $121.00 | |
| Porcine SMC Growth Supplement: Added to Basal Growth Medium to create Growth Medium | Size: 50 ml | CAT.#: P311-GS | Price: $74.00 | |
| Porcine SMC Induction Medium: Provides signals that change cell behavior | Size: 250 ml | CAT.#: P311I-250 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2016
Ameer, G., M. Kibbe and A. Webb. 2016. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20160199535 A1.
Pastrana, H., C. Cooper, M. Alucozai, L. Reece and A. Avila. 2016. Synthesis and in vitro safety assessment of magnetic bacterial cellulose with porcine aortic smooth muscle cells. J Biomed Mat Res, 104:2801-2801.
2014
Gilotti, A. W. Nimlamool, R. Pugh, J. Slee, T. Barthol, E. Miller, and L. Lowe-Krentz. 2014. Heparin Responses in Vascular Smooth Muscle Cells Involve cGMP‐Dependent Protein Kinase (PKG). Journal of Cellular Physiology 229: 2142–2152.
2011
Torrado, M., R. Iglesias, A. Centeno, E. López, and A.T. Mikhailov. 2011. Targeted gene-silencing reveals the functional significance of myocardin signaling in the failing heart. PloS one. 6:e26392.
2010
Blaukovitch, C.I., R. Pugh, A.C. Gilotti, D. Kanyi, and L.J. Lowe‐Krentz. 2010. Heparin treatment of vascular smooth muscle cells results in the synthesis of the dual‐specificity phosphatase MKP‐1. J. cellular biochemistry. 110:382-391.
2009
Ameer, G., M. Kibbe, and A. Webb. 2009. Controlled and Localized Release of Retinoids to Improve Neointimal Hyperplasia. Patent Application US 20100036476 A1.
2008
Allen, J., S. Khan, M.C. Serrano, and G. Ameer. 2008. Characterization of porcine circulating progenitor cells: toward a functional endothelium. Tissue Engineering Part A. 14:183-194.