MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions PCK

Description

Porcine Corneal Keratocytes (PCK) are primary cells derived from porcine cornea. Corneal Keratocytes are neural-crest derived mesenchymal cells that populate the corneal stroma. The stroma is comprised of a type I and type V collagen fibers arranged as lamellae, makes up majority of the corneal thickness responsible for promoting corneal transparency. Upon corneal injury, keratocytes are stimulated to either undergo apoptosis or transition into repair phenotypes to promote corneal regeneration.
 
Porcine Corneal Keratocytes cultured in Corneal Keratocyte Growth Medium are able to propagate to 10 population doublings when cultured under the recommended conditions. PCK could be used as an important in vitro cell model to study keratocyte differentiation, corneal injury and related diseases.
 
 
Porcine Corneal Keratocytes: PCK Top: Phase-contrast image, Bottom Left: PCK immunostained for Vimentin (green), Bottom Right: Fibronectin (green), nuclei are stained with DAPI.

Details

TissueNormal healthy porcine cornea
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 PCK (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen PCK (P632-05), Growth Medium (P6111-500), Subculture Rgnt Kit (090K)
ProliferatingShipped in Tsfr Med, psg 1, flasks or plates
DoublingsAt least 15
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 
Instructions PCK

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved PCK: Frozen PCK (5x10^5) Size: 1 Cryovial CAT.#: P632-05 Price: $679.00
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Cryopreserved PCK: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: P632K-05 Price: $829.00
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Proliferating PCK: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: P633-25 Price: $679.00
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Proliferating PCK: Actively growing, dividing cells in medium  Size: T-75 Flask CAT.#: P633-75 Price: $869.00
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Proliferating PCK: Actively growing, dividing cells in medium   Size: 24 Well CAT.#: P633-24W Price: $869.00
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Proliferating PCK: Actively growing, dividing cells in medium   Size: 96 Well CAT.#: P633-96W Price: $989.00
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Related Products

Product Size CAT.# Price Quantity
PCK Basal Medium: Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: P6110-500 Price: $65.00
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PCK Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: P6111-500 Price: $105.00
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PCK Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: 500 ml CAT.#: P6111K-500 Price: $115.00
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PCK Growth Medium Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: P6111-GS Price: $50.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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