MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions PCK
Description
Porcine Corneal Keratocytes (PCK) are primary cells derived from porcine cornea. Corneal Keratocytes are neural-crest derived mesenchymal cells that populate the corneal stroma. The stroma is comprised of a type I and type V collagen fibers arranged as lamellae, makes up majority of the corneal thickness responsible for promoting corneal transparency. Upon corneal injury, keratocytes are stimulated to either undergo apoptosis or transition into repair phenotypes to promote corneal regeneration.
Porcine Corneal Keratocytes cultured in Corneal Keratocyte Growth Medium are able to propagate to 10 population doublings when cultured under the recommended conditions. PCK could be used as an important in vitro cell model to study keratocyte differentiation, corneal injury and related diseases.
Porcine Corneal Keratocytes: PCK Top: Phase-contrast image, Bottom Left: PCK immunostained for Vimentin (green), Bottom Right: Fibronectin (green), nuclei are stained with DAPI.
Details
| Tissue | Normal healthy porcine cornea |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 PCK (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen PCK (P632-05), Growth Medium (P6111-500), Subculture Rgnt Kit (090K) |
| Proliferating | Shipped in Tsfr Med, psg 1, flasks or plates |
| Doublings | At least 15 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Porcine Corneal Keratocytes Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: P632K-05 | Price: $991.00 | |
| Cryopreserved Porcine Corneal Keratocytes (PCK), adult: Frozen PCK (5x10^5) | Size: 1 Cryovial | CAT.#: P632-05 | Price: $828.00 | |
| Proliferating Porcine Corneal Keratocytes (PCK): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: P633-25 | Price: $828.00 | |
| Proliferating Porcine Corneal Keratocytes (PCK): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: P633-75 | Price: $1,018.00 | |
| Proliferating Porcine Corneal Keratocytes (PCK): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: P633-24W | Price: $1,018.00 | |
| Proliferating Porcine Corneal Keratocytes (PCK): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: P633-96W | Price: $1,138.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Porcine Corneal Keratocyte Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: P6111-500 | Price: $110.00 | |
| Porcine Corneal Keratocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: 500 ml | CAT.#: P6111K-500 | Price: $121.00 | |
| Porcine Corneal Keratocyte Basal Medium : Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: P6110-500 | Price: $72.00 | |
| Porcine Corneal Keratocyte Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: P6111-GS | Price: $65.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
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