MSDS Cryopreserved Cells
Instructions PCAEC
5 Important Cell Culture Rules
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Porcine Coronary Artery Endothelial Cells (PCAEC) provide a useful tool to study artery endothelial function and metabolism. Arterial endothelial cells play critical roles in cardiac homeostasis, and insults to the endothelial cells have been linked to a variety of vascular diseases.
PCAEC from Cell Applications, Inc. have been used to:
- Investigate a new mechanism of obesity-associated vascular disease by demonstrating that resistin, an adipocyte-derived cytokine implemented in insulin resistance and diabetes, can cause endothelial dysfunction in coronary arteries through oxidative stress and down-regulation of NO synthase
- Show that secretoneurin activates expression of NO synthase and calmodulin, leading to endothelial relaxation
- Reveal anti-inflammatory, cardioprotective effects of a novel adenosine-lidocaine drug formulation by observing adhesion and transmigration
(Click to Enlarge) Porcine Coronary Artery Endothelial: PCAEC
Details
Tissue | Normal healthy porcine coronary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | DiI-Ac-LDL uptake: Positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 PAOEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen PCAEC (P300-05), Growth Medium (P211-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 12 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Porcine Coronary Artery Endothelial Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: P300K-05 | Price: $943.00 | |
| Cryopreserved Porcine Coronary Artery Endothelial Cells (PCAEC): Frozen PCAEC (5x10^5) | Size: 1 Cryovial | CAT.#: P300-05 | Price: $775.00 | |
| Proliferating Porcine Coronary Artery Endothelial Cells (PCAEC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: P301-25 | Price: $775.00 | |
| Proliferating Porcine Coronary Artery Endothelial Cells (PCAEC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: P301-75 | Price: $965.00 | |
| Proliferating Porcine Coronary Artery Endothelial Cells (PCAEC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: P301-24W | Price: $965.00 | |
| Proliferating Porcine Coronary Artery Endothelial Cells (PCAEC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: P301-96W | Price: $1,085.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Porcine EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: P210-500 | Price: $70.00 | |
| Porcine EC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: P211-500 | Price: $121.00 | |
| Porcine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: P211K-500 | Price: $130.00 | |
| Porcine EC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: P211-GS | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Publications
2012
Shi, W., R. Jiang, G.P. Dobson, A. Granfeldt, and J. Vinten-Johansen. 2012. The nondepolarizing, normokalemic cardioplegia formulation adenosine-lidocaine (adenocaine) exerts anti-neutrophil effects by synergistic actions of its components. The Journal of Thoracic and Cardiovascular Surgery. 143:1167-1175.
2011
han, C.K., and P.M. Vanhoutte. 2011. Secretoneurin facilitates endothelium-dependent relaxations in porcine coronary arteries. American Journal of Physiology-Heart and Circulatory Physiology. 300:H1159-H1165.
2006
Zhou, W., H. Chai, A. Courson, P. Lin, A. Lumsden, Q. Yao, and C. Chen. 2006. Ginkgolide A attenuates homocysteine-induced endothelial dysfunction in porcine coronary arteries. J Vascular Surg, 44:853-862.
2005
Kougias, P., H. Chai, P.H. Lin, A.B. Lumsden, Q. Yao, and C. Chen. 2005. Adipocyte-derived cytokine resistin causes endothelial dysfunction of porcine coronary arteries. Journal of Vascular Surgery. 41:691-698.