MSDS Cryopreserved Cells
Instructions PPAEC
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Porcine Pulmonary Artery Endothelial Cells (PPAEC) from Cell Applications, Inc. have been used to:

  • Determine that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP
  • This event led to downregulation of Rac1, and induction of stress fibers & focal adhesions via a RhoA/ROCK dependent mechanism
  • The resulting decreased cell motility, and increase in endothelial permeability, linked vascular repair and function with NO production
  • PPAEC have also been used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair

(Click to Enlarge) Porcine Pulmonary Artery Endothelial Cells: PPAEC

Details

Tissue
Normal healthy porcine pulmonary artery
QC
No bacteria, yeast, fungi, mycoplasma
Character
DiI-Ac-LDL uptake: Positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 PPAEC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen PPAEC (P302-05), Growth Medium (P211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions PPAEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved PPAEC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: P302K-05 Price: $778.00
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Cryopreserved PPAEC: Frozen PPAEC (5x10^5) Size: 1 Cryovial CAT.#: P302-05 Price: $630.00
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Proliferating PPAEC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: P303-25 Price: $630.00
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Proliferating PPAEC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: P303-75 Price: $834.00
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Proliferating PPAEC: Actively growing, dividing cells in medium Size: 6 Well CAT.#: P303-6W Price: $834.00
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Proliferating PPAEC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: P303-96W Price: $963.00
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Related Products

Product Size CAT.# Price Quantity
Porcine EC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: P210-500 Price: $54.00
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Porcine EC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: P211-500 Price: $105.00
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Porcine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: P211K-500 Price: $113.00
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Porcine EC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: P211-GS Price: $57.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Airway Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2013
Xiong, Y., W.Y. Chan, A.W. Chua, J. Feng, P. Gopal, Y.S. Ong, and C. Song. 2013. Decellularized Porcine Saphenous Artery for SmallDiameter TissueEngineered Conduit Graft. Artificial organs. 37:E74–E87.
2009
Wojciak-Stothard, B., B. Torondel, L. Zhao, T. Renné, and J.M. Leiper. 2009. Modulation of Rac1 Activity by ADMA/DDAH Regulates Pulmonary Endothelial Barrier Function. Molecular biology of the cell. 20:33-42.
2007
Wojciak-Stothard, B., B. Torondel, L.Y.F. Tsang, I. Fleming, B. Fisslthaler, J.M. Leiper, and P. Vallance. 2007. The ADMA/DDAH pathway is a critical regulator of endothelial cell motility. Journal of cell science. 120:929-942.