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Description
Porcine Pulmonary Artery Endothelial Cells (PPAEC) from Cell Applications, Inc. have been used to:
- Determine that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP
- This event led to downregulation of Rac1, and induction of stress fibers & focal adhesions via a RhoA/ROCK dependent mechanism
- The resulting decreased cell motility, and increase in endothelial permeability, linked vascular repair and function with NO production
- PPAEC have also been used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair
(Click to Enlarge) Porcine Pulmonary Artery Endothelial Cells: PPAEC
Details
Tissue | Normal healthy porcine pulmonary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | DiI-Ac-LDL uptake: Positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 PPAEC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen PPAEC (P302-05), Growth Medium (P211-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Porcine Pulmonary Artery Endothelial Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: P302K-05 | Price: $949.00 | |
| Cryopreserved Porcine Pulmonary Artery Endothelial Cells (PPAEC): Frozen PPAEC (5x10^5) | Size: 1 Cryovial | CAT.#: P302-05 | Price: $775.00 | |
| Proliferating Porcine Pulmonary Artery Endothelial Cells (PPAEC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: P303-25 | Price: $775.00 | |
| Proliferating Porcine Pulmonary Artery Endothelial Cells (PPAEC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: P303-75 | Price: $965.00 | |
| Proliferating Porcine Pulmonary Artery Endothelial Cells (PPAEC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: P303-24W | Price: $965.00 | |
| Proliferating Porcine Pulmonary Artery Endothelial Cells (PPAEC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: P303-96W | Price: $1,085.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Porcine EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: P210-500 | Price: $70.00 | |
| Porcine EC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: P211-500 | Price: $121.00 | |
| Porcine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: P211K-500 | Price: $130.00 | |
| Porcine EC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: P211-GS | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2013
Xiong, Y., W.Y. Chan, A.W. Chua, J. Feng, P. Gopal, Y.S. Ong, and C. Song. 2013. Decellularized Porcine Saphenous Artery for Small‐Diameter Tissue‐Engineered Conduit Graft. Artificial organs. 37:E74–E87.
2009
Wojciak-Stothard, B., B. Torondel, L. Zhao, T. Renné, and J.M. Leiper. 2009. Modulation of Rac1 Activity by ADMA/DDAH Regulates Pulmonary Endothelial Barrier Function. Molecular biology of the cell. 20:33-42.
2007
Wojciak-Stothard, B., B. Torondel, L.Y.F. Tsang, I. Fleming, B. Fisslthaler, J.M. Leiper, and P. Vallance. 2007. The ADMA/DDAH pathway is a critical regulator of endothelial cell motility. Journal of cell science. 120:929-942.