pClone Vectors

The pClone GFP and Luciferase Vectors are designed for high-level expression of GFP or luciferase fusion proteins and for creating GFP or luciferase stable cell lines. These innovative vectors contain optimized CMV promoter-intron sequences for significantly higher constitutive expression levels than other mammalian expression vectors. Each pClone vector contains a multiple cloning site on either side of the reporter gene for creating N- or C-terminal fusion proteins. Also, each vector contains a combination kanamycin/neomycin antibiotic selection gene, which allows for selection in both E. coli and mammalian cells, while minimizing vector size so that transfection efficiency is maximally enhanced. The vectors can be used for either transient transfection or for generating stable cells lines expressing the reporter gene only or your fusion-reporter gene construct.

The pClone Vectors offer the following benefits:

• Modified CMV immediate-early promoter provides high level constitutive expression pomoter in a wide variety of cells and cell types
• Convenient choice of GFP or luciferase reporter genes for control expression experiments
• Two multiple cloning sites (MCS) for cloning gene of choice upstream or downstream of reporter gene (in fusion)
• Kanamycin/neomycin resistance gene provide efficient vector selection in E. coli (with kanamycin sulfate) or mammalian cells (with neomycin sulfate)
• SV40 polyadenylation sign provides for stable mRNA by providing efficient transcription termination and polyadenylation
• pUC origin of replication provides high copy number of vector in E. coli.