Description
The Trypsin/EDTA Solution is specially formulated for use with Cell Applications, Inc. adherent primary cells. We do not recommend the use of non-Cell Applications, Inc. Trypsin/EDTA and reagents for trypsinizing and subculturing procedures.
The Trypsin/EDTA Solution is available as part of a Subculture Reagent Kit, which also contains Hanks Balanced Salt Solution (HBSS) and Trypsin Neutralizing Solution (TNS). The ultra-convenient Subculture Reagent Kit helps ensure the health of your primary cell cultures.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Subculture Reagent Kit
Details
Follow the following important guidelines when subculturing primary cells:
- All reagents should be at room temperature. Do Not Warm Any Reagents to 37 °C.
- Do not over-trypsinize. Strictly follow the trypsinization instructions in our protocol.
- Watch cells under the microscope during the entire trypsinization process. When the cells become rounded but are still attached, hit the side of the flask against your palm until the cells detach.
Learn more about how to properly apply Trpysin/EDTA and maintain the health of your cell cultures in the Cell Culture FAQ.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| HBSS: 1X HBSS for washing of cells before Trypsin/EDTA treatment | Size: 100 ml | CAT.#: 062-100 | Price: $23.00 | |
| Trypsin/EDTA Solution: For subculturing of adherent cells. | Size: 100 ml | CAT.#: 070-100 | Price: $23.00 | |
| Trypsin Neutralization Solution: Stops the trypsinization reaction. | Size: 100 ml | CAT.#: 080-100 | Price: $23.00 |
Publications
2017
Bidarimath, M., K. Khalaj, R. Kridli, F. Kan, M. Koti, and C. Tayade. 2017. Extracellular vesicle mediated intercellular communication at the porcine maternal-fetal interface: A new paradigm for conceptus-endometrial cross-talk. Scientific Reports, doi:10.1038/srep40476.
2016
Bidarimath, M. 2016. Immune-Angiogenesis Mechanisms Associated with Porcine Pregnancy Success and Failure. PhD Thesis, Queen’s University.
Li, H., J. Qian, J. Chen, K. Zhong and S. Chen. 2016. Osteochondral repair with synovial membrane‑derived mesenchymal stem cells. Molecular Medicine Reports, 13:2071-2077.
Ma, L., X. Xun, Y. Qiao, J. An and M. Su. 2016. Predicting efficacies of anticancer drugs using single cell HaloChip assay. Analyst, 141:2454-2462.
Raghavendran, H., S. Mohan, K. Genasan, M. Murali, S. Naveen, S. Talebian, R. McKean and T. Kamarul. 2016. Synergistic interaction of platelet derived growth factor (PDGF) with the surface of PLLA/Col/HA and PLLA/HA scaffolds produces rapid osteogenic differentiation. Colloids and Surfaces B: Biointerfaces, 139:68-78.
Zeimaran, E., S. Mohan, S. Pourshahrestani, B. Pingguan-Murphy, N. Kadri, M. Murali, H. Raghavendran, K. Hasikin, T. Kamarul and M. Towler. 2016. Osteogenic differentiation of mesenchymal stem cells on a poly (octanediol citrate)/bioglass composite scaffold in vitro. Materials & Design, 109:434-442.
2015
de Sá Rodrigues, L., K. Holmes, V. Thompson, M. Newton and T. Stein. 2015. Tumourigenic canine osteosarcoma cell lines associated with frizzled-6 up-regulation and enhanced side population cell frequency. Vet & Comp Oncol, DOI: 10.1111/vco.12138. SC
González-Miguel, J., R. Morchón, E. Carretón, J. Montoya-Alonso and F. Simón. 2015. Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritis? Parasites & Vectors, 8:194.
Puvaneswary, S., S. Talebian, H. Raghavendran, M. Murali, M. Mehrali, A. Afifi, N. Kasim and T. Kamarul. 2015. Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering. Carbohydrate Polymers, 134:799–807.
2014
Pun, P., A. Logan, V. Darley-Usmar, B. Chacko, M. Johnson, G. Huang, S. Rogatti, T. Prime, C. Methner, T. Krieg, I. Fearnley, L. Larsen, D. Larsen, K. Menger, Y. Collins, A. James, G. Kumar, R. Hartley, R. Smith and M. Murphy. 2014. A mitochondria-targeted mass spectrometry probe to detect glyoxals: implications for diabetes. Free Rad Biol & Med, 67:437-450.
Shu, B., J. Xie, Y. Xu, W. Lai, Y. Huang, R. Mao, X. Liu and S Qi. 2014. Effects of skin-derived precursors on wound healing of denervated skin in a nude mouse model. Int J Clin Exp Pathol, 8:2660-2669.
Talebian, S., M. Mehrali, S. Mohan, H. Raghavendran, M. Mehrali, H. Khanlou, T. Kamarul, A. Afifi and A. Abass. 2014. Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering. RSC Adv, 4:49144-49152.
Raghavendran, H., S. Puvaneswary, S. Talebian, M. Murali, S. Naveen, G. Krishnamurithy, R. McKean and T. Karmarul. 2014. A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application.
PLoS ONE, dx.doi.org/10.1371/journal.pone.0104389.
2012
Ohta, K. 2012. Method for producing pluripotent cell using bacterium having fermentation ability. Patent Aplication US 20140255942 A1.
Jin, M., A. Wu, S. Dorzhin, Q. Yue, Y. Ma and D. Liu. 2012. Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization. Cytotechnology, 64:379-389. “Bovine Embryonic Fibroblast GM”??
Liu, D., Y. Lin, T. Kang, B. Huang, W. Xu, M. Garcia-Barrio, M. Olatinwo, R. Mattews, Y. Chen and W. Thompson. 2012. Mitochondrial Dysfunction and Adipogenic Reduction by Prohibitin Silencing in 3T3-L1 Cells. PLoS ONE, dx.doi.org/10.1371/journal.pone.0034315.
Villareal, M., J. Han, K. Ikuta, and H. Isoda. 2012. Mechanism of Mitf inhibition and morphological differentiation effects of hirsein A on B16 melanoma cells revealed by DNA microarray. J Dermatol Sci, 67:26-36.
2011
Nguyen, L., S. Liao, S. Ramakrishna, and C. Chan. 2011. The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage. Nanomedicine, 6:961-974.
Yang, C.J., C.Y. Lin, T.-c. Hsieh, S.C. Olson, and J.M. Wu. 2011. Control of eotaxin-1 expression and release by resveratrol and its metabolites in culture human pulmonary artery endothelial cells. American journal of cardiovascular disease. 1:16.
2010
Wakabayashi, M., Y. Ito, T. Hamazaki and H. Okochi. 2010. Efficient Myogenic Differentiation of Murine Dermal Sca-1 (−) Cells via Initial Aggregation Culture. Tiss Eng Pt A, 16:3251-3259.
2009
Ilagan, B.G., and B.G. Amsden. 2009. Surface modifications of photocrosslinked biodegradable elastomers and their influence on smooth muscle cell adhesion and proliferation. Acta Biomaterialia. 5:2429-2440.