MSDS Cryopreserved Cells
Instructions RbAOSMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rabbit Aortic Smooth Muscle Cells (RbAOSMC) from Cell Applications, Inc. were used to:
  • Demonstrate that anti-restenosis drug probucol inhibits vascular smooth cell proliferation by enhancing apoptosis and inducing heme oxygenase-1
  • Evaluate performance of a novel pH/CO2 sensor system for measuring metabolic activity of cultured cells
  • Characterize the receptor pharmacology, antiplatelet activity, and vascular pharmacology of a novel receptor antagonist
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Rabbit Aortic Smooth Muscle Cells: RbAOSMC

Details

Tissue
Normal healthy rabbit aorta
QC
No bacteria, yeast, fungi, mycoplasma 
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 RbAOSMC (2nd psg) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial RbAOSMC (Rb354-05), Growth Medium (Rb311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 6
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RbAOSMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RbAOSMC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: Rb354K-05 Price: $814.00
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Cryopreserved RbAOSMC: Cryopreserved RbAOSMC (5x10^5) Size: 1 Cryovial CAT.#: Rb354-05 Price: $667.00
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Proliferating RbAOSMC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: Rb355-96W Price: $1,001.00
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Proliferating RbAOSMC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: Rb355-25 Price: $667.00
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Proliferating RbAOSMC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: Rb355-75 Price: $872.00
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Proliferating RbAOSMC: Actively growing, dividing cells in medium Size: 6 Well CAT.#: Rb355-6W Price: $872.00
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Related Products

Product Size CAT.# Price Quantity
Rabbit SMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: Rb310-500 Price: $57.00
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Rabbit SMC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: Rb311-500 Price: $105.00
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Rabbit SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: Rb311K-500 Price: $113.00
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Rabbit SMC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: Rb311-GS Price: $57.00
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Rabbit SMC Induction Medium: Provides signals that change cell behavior Size: 250 ml CAT.#: Rb311I-250 Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2010
Ni, J., A. Waldman, and L. Khachigian. 2010. c-Jun Regulates Shear- and Injury-inducible Egr-1 Expression, Vein Graft Stenosis after Autologous End-to-Side Transplantation in Rabbits, and Intimal Hyperplasia in Human Saphenous Veins. JBC, 285:4038-4048.
Pappan, K. 2010. Hmg-coa secondary metabolites and uses thereof. Patent Application US 20120121554 A1.
2009
Adams, J.W., J. Ramirez, Y. Shi, W. Thomsen, J. Frazer, M. Morgan, J.E. Edwards, W. Chen, B.R. Teegarden, and Y. Xiong. 2009. APD791, 3-methoxy-n-(3-(1-methyl-1h-pyrazol-5-yl)-4-(2-morpholinoethoxy) phenyl) benzamide, a novel 5-hydroxytryptamine 2A receptor antagonist: pharmacological profile, pharmacokinetics, platelet activity and vascular biology. Journal of Pharmacology and Experimental Therapeutics. 331:96-103.
2006
Mohri, S., J. Shimizu, N. Goda, T. Miyasaka, A. Fujita, M. Nakamura, and F. Kajiya. 2006. Measurements of CO2, lactic acid and sodium bicarbonate secreted by cultured cells using a flow-through type pH/CO2 sensor system based on ISFET. Sensors and Actuators B: Chemical. 115:519-525.
2004
Deng, Y.-M., B.J. Wu, P.K. Witting, and R. Stocker. 2004. Probucol Protects Against Smooth Muscle Cell Proliferation by Upregulating Heme Oxygenase-1. Circulation. 110:1855-1860.