MSDS Cryopreserved Cells
Instructions RbAOSMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rabbit Aortic Smooth Muscle Cells (RbAOSMC) from Cell Applications, Inc. were used to:
- Demonstrate that anti-restenosis drug probucol inhibits vascular smooth cell proliferation by enhancing apoptosis and inducing heme oxygenase-1
- Evaluate performance of a novel pH/CO2 sensor system for measuring metabolic activity of cultured cells
- Characterize the receptor pharmacology, antiplatelet activity, and vascular pharmacology of a novel receptor antagonist
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Rabbit Aortic Smooth Muscle Cells: RbAOSMC
Details
Tissue | Normal healthy rabbit aorta |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 RbAOSMC (2nd psg) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial RbAOSMC (Rb354-05), Growth Medium (Rb311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 6 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rabbit Aortic Smooth Muscle Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: Rb354K-05 | Price: $899.00 | |
| Cryopreserved Rabbit Aortic Smooth Muscle Cells (RbAOSMC): Cryopreserved RbAOSMC (5x10^5) | Size: 1 Cryovial | CAT.#: Rb354-05 | Price: $725.00 | |
| Proliferating Rabbit Aortic Smooth Muscle Cells (RbAOSMC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: Rb355-25 | Price: $725.00 | |
| Proliferating Rabbit Aortic Smooth Muscle Cells (RbAOSMC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: Rb355-75 | Price: $915.00 | |
| Proliferating Rabbit Aortic Smooth Muscle Cells (RbAOSMC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: Rb355-24W | Price: $915.00 | |
| Proliferating Rabbit Aortic Smooth Muscle Cells (RbAOSMC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: Rb355-96W | Price: $1,035.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: Rb310-500 | Price: $74.00 | |
| Rabbit SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: Rb311-500 | Price: $121.00 | |
| Rabbit SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: Rb311K-500 | Price: $130.00 | |
| Rabbit SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: Rb311-GS | Price: $74.00 | |
| Rabbit SMC Induction Medium: Provides signals that change cell behavior | Size: 250 ml | CAT.#: Rb311I-250 | Price: $78.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2010
Ni, J., A. Waldman, and L. Khachigian. 2010. c-Jun Regulates Shear- and Injury-inducible Egr-1 Expression, Vein Graft Stenosis after Autologous End-to-Side Transplantation in Rabbits, and Intimal Hyperplasia in Human Saphenous Veins. JBC, 285:4038-4048.
Pappan, K. 2010. Hmg-coa secondary metabolites and uses thereof. Patent Application US 20120121554 A1.
2009
Adams, J.W., J. Ramirez, Y. Shi, W. Thomsen, J. Frazer, M. Morgan, J.E. Edwards, W. Chen, B.R. Teegarden, and Y. Xiong. 2009. APD791, 3-methoxy-n-(3-(1-methyl-1h-pyrazol-5-yl)-4-(2-morpholinoethoxy) phenyl) benzamide, a novel 5-hydroxytryptamine 2A receptor antagonist: pharmacological profile, pharmacokinetics, platelet activity and vascular biology. Journal of Pharmacology and Experimental Therapeutics. 331:96-103.
2006
Mohri, S., J. Shimizu, N. Goda, T. Miyasaka, A. Fujita, M. Nakamura, and F. Kajiya. 2006. Measurements of CO2, lactic acid and sodium bicarbonate secreted by cultured cells using a flow-through type pH/CO2 sensor system based on ISFET. Sensors and Actuators B: Chemical. 115:519-525.
2004
Deng, Y.-M., B.J. Wu, P.K. Witting, and R. Stocker. 2004. Probucol Protects Against Smooth Muscle Cell Proliferation by Upregulating Heme Oxygenase-1. Circulation. 110:1855-1860.