Instructions RA
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat astrocytes (RA) are derived from rat cerebral cortex.  Astrocytes are the most abundant cells in the central nervous system where they perform many functions, such as providing mechanical support and nutrients to neurons and removal of wastes from neurons; providing signaling to endothelial cells; regulating neurogenesis and controlling synaptic function.  As the recognition of the importance of astrocytes in nervous system is increasing, RA serve as a useful in vitro model for exploring the diversity of astrocytes functions.
RA from CAI have been used to:
  • Assess neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke
  • Investigate the effects of astrocytes on brain microvascular endothelial cells in relation to iron transport pathway
Characterization:  Stained positive for GFAP (Example image at right shows neonatal Rat Astrocytes:RAn stained positive for glial fibrillary acidic protein)
(Click to Enlarge) Cryopreserved Rat Astrocytes (RA) revived and cultured in Complete Rat Astrocyte Growth Medium (L). RA stained for GFAP (in red) and DAPI (in blue) (R).

Details

Tissue
Normal healthy Rat brain
QC
No bacteria, yeast, fungi, mycoplasma
Cryovial
500,000 RA (2nd passage) frozen in Basal Medium w/10% FBS, 10% DMSO
Character
Positive for GFAP
Bioassay
Attach, spread, proliferate in Growth Medium
Kit
Cryovial frozen RA (R882A-05n), Growth Med (R821-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RA

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RA, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R882AK-05n Price: $541.00
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Cryopreserved RA, Neonatal: Frozen RA (5x10^5) Size: 1 Cryovial CAT.#: R882A-05n Price: $393.00
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Proliferating RA, Adult: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: R883A-75a Price: $705.00
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Proliferating RA, Adult: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: R883A-96Wa Price: $834.00
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Proliferating RA, Neonatal: Actively growing, dividing cells, in medium Size: 6 Well CAT.#: R883A-6Wn Price: $597.00
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Proliferating RA, Neonatal: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: R883A-75n Price: $597.00
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Proliferating RA, Neonatal: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: R883A-96Wn Price: $726.00
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Proliferating RA, Neonatal: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: R883A-25n Price: $393.00
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Related Products

Product Size CAT.# Price Quantity
Rat Astrocyte Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: R820-500 Price: $67.00
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Rat Astrocyte Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: R821-500 Price: $129.00
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Rat Astrocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: R821K-500 Price: $138.00
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Rat Astrocyte Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: R821-GS Price: $51.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2014
McCarthy, R. and D. Kosman. 2014. Glial Cell Ceruloplasmin and Hepcidin Differentially Regulate Iron Efflux from Brain Microvascular Endothelial CellsPLoS ONE, doi.org/10.1371/journal.pone.0089003.
2013

Liu, S., G. Zhen, R.C. Li, and S. Dore. 2013. Acute bioenergetic intervention or pharmacological preconditioning protects neuron against ischemic injury. Journal of experimental stroke & translational medicine. 6:7-17.

2014
McCarthy, R.C., and D.J. Kosman. 2014. Glial Cell Ceruloplasmin and Hepcidin Differentially Regulate Iron Efflux from Brain Microvascular Endothelial Cells. PloS one. 9:e89003.
2013
Liu, S., G. Zhen, R.C. Li, and S. Dore. 2013. Acute bioenergetic intervention or pharmacological preconditioning protects neuron against ischemic injury. Journal of experimental stroke & translational medicine. 6:7-17.