Instructions RA
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat astrocytes (RA) are derived from rat cerebral cortex. Astrocytes are the most abundant cells in the central nervous system where they perform many functions, such as providing mechanical support and nutrients to neurons and removal of wastes from neurons; providing signaling to endothelial cells; regulating neurogenesis and controlling synaptic function. As the recognition of the importance of astrocytes in nervous system is increasing, RA serve as a useful in vitro model for exploring the diversity of astrocytes functions.
RA from CAI have been used to:
- Assess neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke
- Investigate the effects of astrocytes on brain microvascular endothelial cells in relation to iron transport pathway
Characterization: Stained positive for GFAP (Example image at right shows neonatal Rat Astrocytes:RAn stained positive for glial fibrillary acidic protein)
(Click to Enlarge) Cryopreserved Rat Astrocytes (RA) revived and cultured in Complete Rat Astrocyte Growth Medium (L). RA stained for GFAP (in red) and DAPI (in blue) (R).
Details
Tissue | Normal healthy Rat brain |
QC | No bacteria, yeast, fungi, mycoplasma |
Cryovial | 500,000 RA (2nd passage) frozen in Basal Medium w/10% FBS, 10% DMSO |
Character | Positive for GFAP |
Bioassay | Attach, spread, proliferate in Growth Medium |
Kit | Cryovial frozen RA (R882A-05n), Growth Med (R821-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 10 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Astrocytes Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R882AK-05n | Price: $607.00 | |
| Cryopreserved Rat Astrocytes (RA), neonatal: Frozen RA (5x10^5) | Size: 1 Cryovial | CAT.#: R882A-05n | Price: $425.00 | |
| Proliferating Rat Astrocytes (RA), neonatal: Actively growing, dividing cells, in medium | Size: T-25 Flask | CAT.#: R883A-25n | Price: $425.00 | |
| Proliferating Rat Astrocytes (RA), neonatal: Actively growing, dividing cells, in medium | Size: T-75 Flask | CAT.#: R883A-75n | Price: $615.00 | |
| Proliferating Rat Astrocytes (RA), neonatal: Actively growing, dividing cells, in medium | Size: 24 Well | CAT.#: R883A-24Wn | Price: $615.00 | |
| Proliferating Rat Astrocytes (RA), neonatal: Actively growing, dividing cells, in medium | Size: 96 Well | CAT.#: R883A-96Wn | Price: $735.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Astrocyte Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: R821-500 | Price: $129.00 | |
| Rat Astrocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R821K-500 | Price: $138.00 | |
| Rat Astrocyte Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R820-500 | Price: $87.00 | |
| Rat Astrocyte Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: R821-GS | Price: $66.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Publications
2014
McCarthy, R. and D. Kosman. 2014. Glial Cell Ceruloplasmin and Hepcidin Differentially Regulate Iron Efflux from Brain Microvascular Endothelial Cells. PLoS ONE, doi.org/10.1371/journal.pone.0089003.
2013
Liu, S., G. Zhen, R.C. Li, and S. Dore. 2013. Acute bioenergetic intervention or pharmacological preconditioning protects neuron against ischemic injury. Journal of experimental stroke & translational medicine. 6:7-17.
2014
McCarthy, R.C., and D.J. Kosman. 2014. Glial Cell Ceruloplasmin and Hepcidin Differentially Regulate Iron Efflux from Brain Microvascular Endothelial Cells. PloS one. 9:e89003.
2013
Liu, S., G. Zhen, R.C. Li, and S. Dore. 2013. Acute bioenergetic intervention or pharmacological preconditioning protects neuron against ischemic injury. Journal of experimental stroke & translational medicine. 6:7-17.