Instructions RCoN R822N-20
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Brochure Cell Applications Inc
Description
Rat cortical neurons (RCoN) are derived from cerebral cortices of day 18 embryonic Sprague Dawley rat brains. When cultured under the recommended conditions, RCoN arborize and form complex neurite network in one week. RCoN Stain positive for β III-Tubulin.
The cerebral cortex plays a key role in cognition, memory, attention, perceptual awareness, thought, language and consciousness. Neurons connect to other neural cells through neurites and synapses to form complex neurocircuitry for signal transmission. A healthy circuitry in the brain is essential for cognitive functions, proper control and regulation of the other parts of the body. Dysfunction and degeneration in the cerebral cortex have been implicated in various brain injuries, neurological and psychiatric disorders. Cortical neurons, therefore, provide an excellent cellular model system to study disease mechanisms and pathophysiologies. They also serve as a platform for drug screening and development, toxicity tests, immunostaining, live cell imaging, co-culturing and electrophysiology.
(Click to Enlarge) Freshly isolated Rat Cortical Neurons (RCoN) cultured for 5 days (A). Freshly isolated RCoN (B), and revived cryopreserved RCoN (C) stained on day 8 for β III-Tubulin (green), GFAP (red), and DAPI (blue).
Details
| Tissue | Normal healthy rat brain cortex |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for β-III Tubulin |
| Bioassay | Plate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med |
| Cryovial | 2M RCoN cryopreserved after isolation in Serum-Free Freezing Medium (042-50) |
| Kit (2M) | 2M cryopreserved RCoN (R882N-20), Coating Soln I (027-05), Plating Med (R817P-10), Culture Med (R817-100) |
| Doublings | N/A - Neurons don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Cortical Neurons Total Kit: 2x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: R882NK-20 | Price: $550.00 | |
| Cryopreserved Rat Cortical Neurons (RCoN): Frozen RCoN (2x10^6) | Size: 1 Cryovial | CAT.#: R882N-20 | Price: $425.00 | |
| Pre-plated from Cryopreserved Rat Cortical Neurons (RCoN): Plated RCoN from cryopreserved vials | Size: 24 Well | CAT.#: R883N-24W | Price: $645.00 | |
| Pre-plated from Cryopreserved Rat Cortical Neurons (RCoN): Plated RCoN from cryopreserved vials | Size: 96 Well | CAT.#: R883N-96W | Price: $436.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Neuron Culture Medium: For the culture and maintenance of neurons. (For rat dorsal root gangion neurons, use Cat# R823K-100.) | Size: 100 ml | CAT.#: R817-100 | Price: $72.00 | |
| Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately. (For rat dorsal root gangion neurons, use Cat# R823K-100.) | Size: Yields 100 ml | CAT.#: R817K-100 | Price: $83.00 | |
| Rat Neuron Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 100 ml | CAT.#: R816-100 | Price: $40.00 | |
| Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium | Size: 3 ml | CAT.#: R817-GS | Price: $57.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 5 ml | CAT.#: 027-05 | Price: $18.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 10 ml | CAT.#: 027-10 | Price: $36.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 20 ml | CAT.#: R817P-20 | Price: $45.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 40 ml | CAT.#: R817P-40 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $465.00 | |
| Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $62.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 |
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