Instructions RCoN R822N-20
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Brochure Cell Applications Inc

Description

Rat cortical neurons (RCoN) are derived from cerebral cortices of day 18 embryonic Sprague Dawley rat brains. When cultured under the recommended conditions, RCoN arborize and form complex neurite network in one week. RCoN Stain positive for β III-Tubulin.
The cerebral cortex plays a key role in cognition, memory, attention, perceptual awareness, thought, language and consciousness. Neurons connect to other neural cells through neurites and synapses to form complex neurocircuitry for signal transmission. A healthy circuitry in the brain is essential for cognitive functions, proper control and regulation of the other parts of the body. Dysfunction and degeneration in the cerebral cortex have been implicated in various brain injuries, neurological and psychiatric disorders. Cortical neurons, therefore, provide an excellent cellular model system to study disease mechanisms and pathophysiologies.  They also serve as a platform for drug screening and development, toxicity tests, immunostaining, live cell imaging, co-culturing and electrophysiology.
(Click to Enlarge) Freshly isolated Rat Cortical Neurons (RCoN) cultured for 5 days (A). Freshly isolated RCoN (B), and revived cryopreserved RCoN (C) stained on day 8 for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat  brain cortex   
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial2M RCoN cryopreserved after isolation in Serum-Free Freezing Medium (042-50)
Kit (2M)2M cryopreserved RCoN (R882N-20), Coating Soln I (027-05), Plating Med (R817P-10), Culture Med (R817-100)
DoublingsN/A - Neurons don't proliferate in vitro
Applications Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
  
  
  
Instructions RCoN R822N-20

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved Rat Cortical Neurons Total Kit: 2x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R882NK-20 Price: $550.00
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Cryopreserved Rat Cortical Neurons (RCoN): Frozen RCoN (2x10^6) Size: 1 Cryovial CAT.#: R882N-20 Price: $425.00
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Pre-plated from Cryopreserved Rat Cortical Neurons (RCoN): Plated RCoN from cryopreserved vials Size: 24 Well CAT.#: R883N-24W Price: $645.00
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Pre-plated from Cryopreserved Rat Cortical Neurons (RCoN): Plated RCoN from cryopreserved vials Size: 96 Well CAT.#: R883N-96W Price: $436.00
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Related Products

Product Size CAT.# Price Quantity
Rat Neuron Culture Medium: For the culture and maintenance of neurons. (For rat dorsal root gangion neurons, use Cat# R823K-100.) Size: 100 ml CAT.#: R817-100 Price: $72.00
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately. (For rat dorsal root gangion neurons, use Cat# R823K-100.) Size: Yields 100 ml CAT.#: R817K-100 Price: $83.00
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $40.00
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $18.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $36.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 20 ml CAT.#: R817P-20 Price: $45.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 40 ml CAT.#: R817P-40 Price: $71.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $465.00
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Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $62.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00

Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Nervous System

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Cell Apps Flyer Brain Cells

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Cell Apps Poster Primary Cells

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