Instructions RDF
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skin Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Dermal Fibroblasts (RDF) from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration.  The cells were also used in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing, and as a control in engineered cardiac tissues.
(Click to Enlarge) Rat Dermal Fibroblasts: RDF.  Isolated from rodent skin tissue.

Details

Tissue
Normal healthy rat dermis
QC
No bacteria, yeast, fungi, mycoplasma
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 RDF (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen RDF (R106-05n), Growth Medium (R116-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, psg 2 (flasks or plates)
Doublings
At least 8
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RDF

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RDF, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R106K-05a Price: $604.00
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Cryopreserved RDF, Adult: Frozen RDF (5x10^5) Size: 1 Cryovial CAT.#: R106-05a Price: $457.00
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Cryopreserved RDF, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R106K-05n Price: $552.00
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Cryopreserved RDF, Neonatal: Frozen RDF (5x10^5) Size: 1 Cryovial CAT.#: R106-05n Price: $404.00
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Proliferating RDF, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R107-25a Price: $425.00
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Proliferating RDF, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R107-75a Price: $615.00
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Proliferating RDF, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: R107-6Wa Price: $615.00
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Proliferating RDF, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R107-96Wa Price: $735.00
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Proliferating RDF, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R107-96Wn Price: $737.00
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Proliferating RDF, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R107-25n Price: $404.00
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Proliferating RDF, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R107-75n Price: $608.00
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Proliferating RDF, Neonatal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: R107-6Wn Price: $608.00
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Related Products

Product Size CAT.# Price Quantity
Rat Fibroblast Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: R115-500 Price: $57.00
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Rat Fibroblast Basal Medium wo Phenol Red: Basal medium (contains no growth supplement). Add GS before use. Without Phenol Red. Size: 500 ml CAT.#: R115PR-500 Price: $66.00
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Rat Fibroblast Growth Medium: All-in-one ready-to-use media Size: 500 ml CAT.#: R116-500 Price: $99.00
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Rat Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: R116K-500 Price: $108.00
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Rat Fibroblast Growth Medium wo FBS: Growth Medium without FBS Size: 500 ml CAT.#: R116F-500 Price: $114.00
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Rat Fibroblast Growth Medium wo Phenol Red: All-in-one ready-to-use media, without Phenol Red Size: 500 ml CAT.#: R116PR-500 Price: $114.00
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Rat Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: R116-GS Price: $51.00
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Rat Fibroblast Growth Supplement wo FBS: Growth Supplement without FBS Size: 50 ml CAT.#: R116F-GS Price: $58.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Fibroblast Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF103KS Price: $54.00
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Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF103K Price: $404.00
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Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 Size: 10 ug CAT.#: RP3027-10 Price: $194.00
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Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 Size: 100 ug CAT.#: RP3027-100 Price: $484.00
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Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 Size: 1000 ug CAT.#: RP3027-1000 Price: $4,090.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Skin Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

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Publications

2017

Uchinaka A, Kawaguchi N, Ban T, Hamada Y, Mori S, Maeno Y, Sawa Y, Nagata K, Yamamoto H. 2017. Evaluation of dermal wound healing activity of synthetic peptide SVVYGLR. Biochem Biophys Res Commun. 2017 Jul 24. pii: S0006-291X(17)31482-1.

Fuji, M., K. Yamanouchi, Y. Saka, Z. Baimakhanov, I. Yamaguchi, A. Soyam, M. Hidka, M. Takatsuki, T. Kurokiand S. Eguchi. 2017. In vivo construction of liver tissue by implantation of a hepatic non-parenchymal/adipose-derived stem cell sheet. J. Tiss Eng & Regen Med, DOI:10.1002/term.2424.
2016
Rathinasabapathy, A., E. Bruce, A. Espejo, A. Horowitz, D. Sudhan, A. Nair, D. Guzzo, J. Francis, M. Raizada, V. Shenoy and M. Katovich. 2016. Therapeutic potential of adipose stem cell-derived conditioned medium against pulmonary hypertension and lung fibrosis. British J Pharmacol, 173:2859-2879.
2015
Aalbers, C., L. Bevaart, S. Loiler, K. de Cortie, J. Wright, F. Mingozzi, P. Tak and M. Vervoordeldonk. 2015. Preclinical Potency and Biodistribution Studies of an AAV 5 Vector Expressing Human Interferon-β (ART-I02) for Local Treatment of Patients with Rheumatoid Arthritis. PLoS ONE 10(6): e0130612.
Hiroharu, O., H. Ryusuke, O. Satoshi, F. Yuki, L. Xing, T. Michiaki, K. Mika and K. Yukinari. 2015. Development of Sensitive Monoclonal Antibody PMab-2 Against Rat Podoplanin. mAbs in Immunodiag & Immunother, 34:396-403. RDF
Uchinaka, A., Y. Hamada, S. Mori, S. Miyagawa, A. Saito, Y. Sawa, N. Matsuura, H. Yamamoto and N. Kawaguchi. 2015. SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis.Molec & Cell Biochem, 408:191-203.
2014
Fei, R., H. Hou, D. Munoz-Pinto, A. Han, M. Hahn, and M. Grunlan. 2014. Thermoresponsive Double Network Micropillared Hydrogels for Controlled Cell Release. Macromolecular Bioscience, 14:1346-1352.
Yan, P., X. Min, Z. Jiping, L. Hongwei, Z. Ziyuan, W. Yu and C. Biao. 2014. Freeze-Dried Rat Bone Marrow Mesenchymal Stem Cell Paracrine Factors: A Simplified Novel Material for Skin Wound Therapy. Tissue Eng Pt A, 21:1036-1046.
2013
Muraoka, I., M. Takatsuki, Y. Sakai, T. Tomonaga, A. Soyama, M. Hidaka, Y. Hishikawa, T. Koji, R. Utoh, and K. Ohashi. 2013. Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model. Journal of tissue engineering and regenerative medicine. doi: 10.1002/term.1718.
2012
Nomoto, Y., W. Okano, M. Imaizumi, A. Tani, M. Nomoto, and K. Omori. 2012. Bioengineered prosthesis with allogenic heterotopic fibroblasts for cricoid regeneration. The Laryngoscope. 122:805-809.
Serrao, G.W., I.C. Turnbull, D. Ancukiewicz, D.E. Kim, E. Kao, T.J. Cashman, L. Hadri, R.J. Hajjar, and K.D. Costa. 2012. Myocyte-depleted engineered cardiac tissues support therapeutic potential of mesenchymal stem cells. Tissue Engineering Part A. 18:1322-1333.