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Description
Rat Mesenchymal Stem Cells (RMSC) have the potential to maintain multipotency and proliferate extensively in vitro. Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages.
RMSC from Cell Applications, Inc. have been used to demonstrate:
- TGF-β stimulates production of MCP-1 by vascular smooth muscle cells, which attracts bone marrow stromal cells
- Cell migration can be stimulated by VPA and lithium through HDAC-CXCR4 and GSK-3β-MMP-9, respectively
- Therapeutic potential of marrow stromal stem cells depending on the extracellular matrix properties
- Combination of low-level laser therapy and transplantation of marrow stromal stem cells results in greater functional recovery after nerve crush injury
- Direct stem cell differentiation by altering physical topography of the substrate
- Surface materials to control cell-adhesion properties
(Click to Enlarge) Rat Mesenchymal Stem Cells: RMSC. Isolated from the bone marrow, these cells support hematopoietic growth and differentiation, and can be induced to produce bone, cartilage, fat, neurons and hepatocytes.
Details
Tissue: | Normal healthy rat bone marrow |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Accumulate lipid in Differentiation Med |
Bioassay | Attach, spread proliferate in Growth Med |
Cryovial | 500,000 RMSC (2nd passage) in RMSC Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen RMSC (R492-05a), Growth Medium (R419-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
Doublings | At least 10 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Mesenchymal Stem Cell Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R492K-05a | Price: $611.00 | |
| Cryopreserved Rat Mesenchymal Stem Cells (RMSC), adult: Frozen RMSC (5x10^5) | Size: 1 Cryovial | CAT.#: R492-05a | Price: $425.00 | |
| Proliferating Rat Mesenchymal Stem Cells (RMSC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R493-25a | Price: $425.00 | |
| Proliferating Rat Mesenchymal Stem Cells (RMSC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R493-75a | Price: $615.00 | |
| Proliferating Rat Mesenchymal Stem Cells (RMSC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R493-96Wa | Price: $735.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| RMSC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R418-500 | Price: $81.00 | |
| RMSC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: R419-500 | Price: $133.00 | |
| RMSC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: R419K-500 | Price: $144.00 | |
| RMSC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 40 ml | CAT.#: R419-GS | Price: $81.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2016
Parashurama, N. B. Ahn, K. Ziv, K. Ito, R. Paulmurugan, J. Willmann, J. Chung, F. Ikeno, J. Swanson, D. Merk, J. Lyons, D. Yerushalmi, T. Teramoto, H. Kosuge, C. Dao, P. Ray, M. Patel, Y. Chang, M. Mahmoudi, J. Cohen, A. Goldstone, F. Habte, S. Bhaumik, S. Yaghoubi, R. Robbins, R. Dash, P. Yang, T. Brinton, P. Yock, M. McConnell and S. Gambhir. 2016. Multimodality Molecular Imaging of Cardiac Cell Transplantation: Part I. Reporter Gene Design, Characterization, and Optical in Vivo Imaging of Bone Marrow Stromal Cells after Myocardial Infarction. Radiology, 280:815-825.
2015
Gershlak, J. and L. Black. 2015. Beta 1 integrin binding plays a role in the constant traction force generation in response to varying stiffness for cells grown on mature cardiac extracellular matrix. Experimental Cell Research, 330:311-324.
Yang, C., J. Wang, S. Chen, Y. Jan, and Y. Hsieh. 2015. Enhanced functional recovery from sciatic nerve crush injury through a combined treatment of cold-water swimming and mesenchymal stem cell transplantation. Neurological Research, DOI: http://dx.doi.org/10.1179/1743132815Y.0000000060.
2014
Neculaes, V., K. Conway, A. Gerner, E. Loghin, S. Yazdanfar, D. Dylov, B. Davis, and C. Joo. 2014. Optical based delivery of exogenous molecules to cells. Patent US 8778682 B2.
Sullivan, K., K. Quinn, K. Tang, I. Georgakoudi and L. Black. 2014. Extracellular matrix remodeling following myocardial infarction influences the therapeutic potential of mesenchymal stem cells. Stem Cell Res & Ther, 5:14.
Ziv, K., H. Nuhn, Y. Haim, L. Sasportas, P. Kempen, T. Niedringhaus, M. Hrynyk, R. Sinclair, A. Barron and S. Gambhir. 2014. A tunable silk–alginate hydrogel scaffold for stem cell culture and transplantation. Biomaterials, 35:3736-3743.
2013
Gershlak, J.R., J.I.N. Resnikoff, K.E. Sullivan, C. Williams, R.M. Wang, and L.D. Black Iii. 2013. Mesenchymal stem cells ability to generate traction stress in response to substrate stiffness is modulated by the changing extracellular matrix composition of the heart during development. Biochemical & biophysical research comm. 439:161-166.
Yang, C.C., J. Wang, S.C. Chen, and Y.L. Hsieh. 2013. Synergistic effects of low‐level laser and mesenchymal stem cells on functional recovery in rats with crushed sciatic nerves. J. Tissue Eng. & Regen. Medicine. doi: 10.1002/term.1714
2011
Brammer, K.S., C. Choi, C.J. Frandsen, S. Oh, and S. Jin. 2011. Hydrophobic nanopillars initiate mesenchymal stem cell aggregation and osteo-differentiation. Acta Biomaterialia. 7:683-690.
Sommani, P., H. Tsuji, H. Sato, Y. Gotoh, and G. Takaoka. 2011. Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion Implantation. Transactions of the Materials Research Society of Japan, 36:317-320.
Tsuji, H., P. Sommani, Y. Hayashi, H. Kojima, H. Sato, Y. Gotoh, G. Takaoka, and J. Ishikawa. 2011. Surface modification of silica glass by CHF3 plasma treatment and carbon negative-ion implantation for cell pattern adhesion. Surface and Coatings Technology. 206:900-904.
2010
Sommani, P., H. Tsuji, H. Kojima, H. Sato, Y. Gotoh, J. Ishikawa, and G.H. Takaoka. 2010. Irradiation effect of carbon negative-ion implantation on polytetrafluoroethylene for controlling cell-adhesion property. Nuclear Instruments and Methods in Physics Research Section B. 268:3231-3234.
Tsai, L.-K., Y. Leng, Z. Wang, P. Leeds, and D.-M. Chuang. 2010. The Mood Stabilizers Valproic Acid and Lithium Enhance Mesenchymal Stem Cell Migration via Distinct Mechanisms. Neuropsychopharmacology. 35:2225-2237.
2009
Zhang, F., S. Tsai, K. Kato, D. Yamanouchi, C. Wang, S. Rafii, B. Liu, and K.C. Kent. 2009. Transforming Growth Factor-β Promotes Recruitment of Bone Marrow Cells and Bone Marrow-derived Mesenchymal Stem Cells through Stimulation of MCP-1 Production in Vascular Smooth Muscle Cells. J. Biological Chemistry. 284:17564-17574.