Instructions RMSC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Mesenchymal Stem Cells (RMSC) have the potential to maintain multipotency and proliferate extensively in vitro. Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages. 
RMSC from Cell Applications, Inc. have been used to demonstrate:
  • TGF-β stimulates production of MCP-1 by vascular smooth muscle cells, which attracts bone marrow stromal cells
  • Cell migration can be stimulated by VPA and lithium through HDAC-CXCR4 and GSK-3β-MMP-9, respectively
  • Therapeutic potential of marrow stromal stem cells depending on the extracellular matrix properties
  • Combination of low-level laser therapy and transplantation of marrow stromal stem cells results in greater functional recovery after nerve crush injury
  • Direct stem cell differentiation by altering physical topography of the substrate
  • Surface materials to control cell-adhesion properties
(Click to Enlarge) Rat Mesenchymal Stem Cells: RMSC.  Isolated from the bone marrow, these cells support hematopoietic growth and differentiation, and can be induced to produce bone, cartilage, fat, neurons and hepatocytes.

Details

Tissue:
Normal healthy rat bone marrow
QC
No bacteria, yeast, fungi, mycoplasma
Character
Accumulate lipid in Differentiation Med
Bioassay
Attach, spread proliferate in Growth Med
Cryovial
500,000 RMSC (2nd passage) in RMSC Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen RMSC (R492-05a), Growth Medium (R419-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RMSC

Format: PDF

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RMSC Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R492K-05a Price: $589.73
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Cryopreserved RMSC, adult: Frozen RMSC (5x10^5) Size: 1 Cryovial CAT.#: R492-05a Price: $425.12
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Proliferating RMSC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R493-25a Price: $425.12
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Proliferating RMSC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: R493-6Wa Price: $629.61
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Proliferating RMSC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R493-75a Price: $629.61
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Proliferating RMSC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R493-96Wa Price: $758.76
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Related Products

Product Size CAT.# Price Quantity
Rat Mesenchymal Stem Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: R418-500 Price: $62.42
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Rat Mesenchymal Stem Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: R419-500 Price: $116.24
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Rat Mesenchymal Stem Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: R419K-500 Price: $124.85
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Rat Mesenchymal Stem Cell Growth Supplements: Added to Basal Medium to create Growth Medium Size: 10 ml CAT.#: R419-GS Price: $62.42
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Skeletal System Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2016
Parashurama, N. B. Ahn, K. Ziv, K. Ito, R. Paulmurugan, J. Willmann, J. Chung, F. Ikeno, J. Swanson, D. Merk, J. Lyons, D. Yerushalmi, T. Teramoto, H. Kosuge, C. Dao, P. Ray, M. Patel, Y. Chang, M. Mahmoudi, J. Cohen, A. Goldstone, F. Habte, S. Bhaumik, S. Yaghoubi, R. Robbins, R. Dash, P. Yang, T. Brinton, P. Yock, M. McConnell and S. Gambhir. 2016. Multimodality Molecular Imaging of Cardiac Cell Transplantation: Part I. Reporter Gene Design, Characterization, and Optical in Vivo Imaging of Bone Marrow Stromal Cells after Myocardial InfarctionRadiology, 280:815-825.
2015
Gershlak, J. and L. Black. 2015. Beta 1 integrin binding plays a role in the constant traction force generation in response to varying stiffness for cells grown on mature cardiac extracellular matrix. Experimental Cell Research, 330:311-324.
2014
Neculaes, V., K. Conway, A. Gerner, E. Loghin, S. Yazdanfar, D. Dylov, B. Davis, and C. Joo. 2014. Optical based delivery of exogenous molecules to cells. Patent US 8778682 B2.
Sullivan, K., K. Quinn, K. Tang, I. Georgakoudi and L. Black. 2014. Extracellular matrix remodeling following myocardial infarction influences the therapeutic potential of mesenchymal stem cells. Stem Cell Res & Ther, 5:14.
Ziv, K., H. Nuhn, Y. Haim, L. Sasportas, P. Kempen, T. Niedringhaus, M. Hrynyk, R. Sinclair, A. Barron and S. Gambhir. 2014. A tunable silk–alginate hydrogel scaffold for stem cell culture and transplantation. Biomaterials, 35:3736-3743.
2013
Gershlak, J.R., J.I.N. Resnikoff, K.E. Sullivan, C. Williams, R.M. Wang, and L.D. Black Iii. 2013. Mesenchymal stem cells ability to generate traction stress in response to substrate stiffness is modulated by the changing extracellular matrix composition of the heart during development. Biochemical & biophysical research comm. 439:161-166.
Yang, C.C., J. Wang, S.C. Chen, and Y.L. Hsieh. 2013. Synergistic effects of lowlevel laser and mesenchymal stem cells on functional recovery in rats with crushed sciatic nerves. J. Tissue Eng. & Regen. Medicine. doi: 10.1002/term.1714
2011
Brammer, K.S., C. Choi, C.J. Frandsen, S. Oh, and S. Jin. 2011. Hydrophobic nanopillars initiate mesenchymal stem cell aggregation and osteo-differentiation. Acta Biomaterialia. 7:683-690.
Sommani, P., H. Tsuji, H. Sato, Y. Gotoh, and G. Takaoka. 2011. Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion Implantation. Transactions of the Materials Research Society of Japan, 36:317-320.
Tsuji, H., P. Sommani, Y. Hayashi, H. Kojima, H. Sato, Y. Gotoh, G. Takaoka, and J. Ishikawa. 2011. Surface modification of silica glass by CHF3 plasma treatment and carbon negative-ion implantation for cell pattern adhesion. Surface and Coatings Technology. 206:900-904.
2010
Sommani, P., H. Tsuji, H. Kojima, H. Sato, Y. Gotoh, J. Ishikawa, and G.H. Takaoka. 2010. Irradiation effect of carbon negative-ion implantation on polytetrafluoroethylene for controlling cell-adhesion property. Nuclear Instruments and Methods in Physics Research Section B. 268:3231-3234.
Tsai, L.-K., Y. Leng, Z. Wang, P. Leeds, and D.-M. Chuang. 2010. The Mood Stabilizers Valproic Acid and Lithium Enhance Mesenchymal Stem Cell Migration via Distinct Mechanisms. Neuropsychopharmacology. 35:2225-2237.
2009
Zhang, F., S. Tsai, K. Kato, D. Yamanouchi, C. Wang, S. Rafii, B. Liu, and K.C. Kent. 2009. Transforming Growth Factor-β Promotes Recruitment of Bone Marrow Cells and Bone Marrow-derived Mesenchymal Stem Cells through Stimulation of MCP-1 Production in Vascular Smooth Muscle Cells. J. Biological Chemistry. 284:17564-17574.