5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Rat Mesenchymal Stem Cells (RMSC) have the potential to maintain multipotency and proliferate extensively in vitro. Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages.
RMSC from Cell Applications, Inc. have been used to demonstrate:
- TGF-β stimulates production of MCP-1 by vascular smooth muscle cells, which attracts bone marrow stromal cells
- Cell migration can be stimulated by VPA and lithium through HDAC-CXCR4 and GSK-3β-MMP-9, respectively
- Therapeutic potential of marrow stromal stem cells depending on the extracellular matrix properties
- Combination of low-level laser therapy and transplantation of marrow stromal stem cells results in greater functional recovery after nerve crush injury
- Direct stem cell differentiation by altering physical topography of the substrate
- Surface materials to control cell-adhesion properties
Normal healthy rat bone marrow
No bacteria, yeast, fungi, mycoplasma
|Accumulate lipid in Differentiation Med|
Attach, spread proliferate in Growth Med
500,000 RMSC (2nd passage) in RMSC Basal Medium w/ 10% FBS, 10% DMSO
Cryovial frozen RMSC (R492-05a), Growth Medium (R419-500), Subcltr Rgnt Kit (090K)
Shipped in Gr Med, 2nd psg (flasks or plates)
|At least 10|
|Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.|
|Cryopreserved RMSC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: R492K-05a||Price: $586.00|
|Cryopreserved RMSC, Adult: Frozen RMSC (5x10^5)||Size: 1 Cryovial||CAT.#: R492-05a||Price: $425.00|
|Proliferating RMSC, Adult: Actively growing, dividing cells in medium||Size: T-25 Flask||CAT.#: R493-25a||Price: $425.00|
|Proliferating RMSC, Adult: Actively growing, dividing cells in medium||Size: 6 Well||CAT.#: R493-6Wa||Price: $630.00|
|Proliferating RMSC, Adult: Actively growing, dividing cells in medium||Size: T-75 Flask||CAT.#: R493-75a||Price: $630.00|
|Proliferating RMSC, Adult: Actively growing, dividing cells in medium||Size: 96 Well||CAT.#: R493-96Wa||Price: $759.00|
|RMSC Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: R418-500||Price: $62.00|
|RMSC Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: R419-500||Price: $116.00|
|RMSC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500ml||CAT.#: R419K-500||Price: $125.00|
|RMSC Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 40 ml||CAT.#: R419-GS||Price: $62.00|
Extended Family Products
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $55.00|
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Sullivan, K., K. Quinn, K. Tang, I. Georgakoudi and L. Black. 2014. Extracellular matrix remodeling following myocardial infarction influences the therapeutic potential of mesenchymal stem cells. Stem Cell Res & Ther, 5:14.
Ziv, K., H. Nuhn, Y. Haim, L. Sasportas, P. Kempen, T. Niedringhaus, M. Hrynyk, R. Sinclair, A. Barron and S. Gambhir. 2014. A tunable silk–alginate hydrogel scaffold for stem cell culture and transplantation. Biomaterials, 35:3736-3743.
Gershlak, J.R., J.I.N. Resnikoff, K.E. Sullivan, C. Williams, R.M. Wang, and L.D. Black Iii. 2013. Mesenchymal stem cells ability to generate traction stress in response to substrate stiffness is modulated by the changing extracellular matrix composition of the heart during development. Biochemical & biophysical research comm. 439:161-166.
Yang, C.C., J. Wang, S.C. Chen, and Y.L. Hsieh. 2013. Synergistic effects of low‐level laser and mesenchymal stem cells on functional recovery in rats with crushed sciatic nerves. J. Tissue Eng. & Regen. Medicine. doi: 10.1002/term.1714
Brammer, K.S., C. Choi, C.J. Frandsen, S. Oh, and S. Jin. 2011. Hydrophobic nanopillars initiate mesenchymal stem cell aggregation and osteo-differentiation. Acta Biomaterialia. 7:683-690.
Sommani, P., H. Tsuji, H. Sato, Y. Gotoh, and G. Takaoka. 2011. Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion Implantation. Transactions of the Materials Research Society of Japan, 36:317-320.
Tsuji, H., P. Sommani, Y. Hayashi, H. Kojima, H. Sato, Y. Gotoh, G. Takaoka, and J. Ishikawa. 2011. Surface modification of silica glass by CHF3 plasma treatment and carbon negative-ion implantation for cell pattern adhesion. Surface and Coatings Technology. 206:900-904.
Sommani, P., H. Tsuji, H. Kojima, H. Sato, Y. Gotoh, J. Ishikawa, and G.H. Takaoka. 2010. Irradiation effect of carbon negative-ion implantation on polytetrafluoroethylene for controlling cell-adhesion property. Nuclear Instruments and Methods in Physics Research Section B. 268:3231-3234.
Tsai, L.-K., Y. Leng, Z. Wang, P. Leeds, and D.-M. Chuang. 2010. The Mood Stabilizers Valproic Acid and Lithium Enhance Mesenchymal Stem Cell Migration via Distinct Mechanisms. Neuropsychopharmacology. 35:2225-2237.
Zhang, F., S. Tsai, K. Kato, D. Yamanouchi, C. Wang, S. Rafii, B. Liu, and K.C. Kent. 2009. Transforming Growth Factor-β Promotes Recruitment of Bone Marrow Cells and Bone Marrow-derived Mesenchymal Stem Cells through Stimulation of MCP-1 Production in Vascular Smooth Muscle Cells. J. Biological Chemistry. 284:17564-17574.