Instructions RMcg
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Microglia (RMcg) are derived from the brain of normal postnatal day 1 rats by standardized methods. When revived and plated and cultured under recommended conditions, RMcg form adherent culture.
Microglia, originated from the hematopoietic stem cells in bone marrow, are the resident macrophages in the central nervous system (CNS) which is separated from the rest of the body due to the presence of blood-brain barrier. Microglia actively survey the surrounding area and respond by scavenging damaged neural cells, plaques, and infectious agents. Due to their functions in immune response and maintaining homeostasis in the CNS, microglia have been implicated in neurodevelopment, CNS plasticity and repair, neuroinflammation, aging and neurodegeneration, neuropathic pain, and infections.
Characterization: RMcg stain positive for F4/80 and CD11b/c, and uptake DiI-Ac-LDL
(Click to Enlarge) Rat Microglia Cells (RMcg) cultured 3 days (Top) and stained (Bottom) for CD11b/c (green) F4/80 (red), DAPI (blue) (L) and DiI-Ac-LDL (red) uptake (R).
Details
| Tissue | Normal, healthy Rat brain |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for F4/80 and CD11b/c |
| Bioassay | Plate, spread on CC ware & uptake DiI-Ac-LDL |
| Cryovial | 1M RMcg frozen in Macrophage & Microglia Cell Freezing Med (043-50) |
| Kit | Cryovial Frozen RMcg (R8816K-10n), Macrophage Cltr Med Kit (R619K-100), Coating Soln (1021-5) |
| Doublings | N/A - Microglia Cells don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Microglia Total Kit, neonatal: 1x10^6 Cells (Neonatal) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: R8816K-10n | Price: $625.00 | |
| Cryopreserved Rat Microglia Cells (RMcg), neonatal: Frozen RMcg (1x10^6) | Size: 1 Cryovial | CAT.#: R8816-10n | Price: $550.00 | |
| Cultured Rat Microglia Cells (RMcg), neonatal: Cultured cells, in medium | Size: T-25 Flask | CAT.#: R8817-25n | Price: $550.00 | |
| Cultured Rat Microglia Cells (RMcg), neonatal: Cultured cells, in medium | Size: T-75 Flask | CAT.#: R8817-75n | Price: $740.00 | |
| Cultured Rat Microglia Cells (RMcg), neonatal: Cultured cells in medium | Size: 24 Well | CAT.#: R8817-24Wn | Price: $740.00 | |
| Cultured Rat Microglia Cells (RMcg), neonatal: Cultured cells in medium | Size: 96 Well | CAT.#: R8817-96Wn | Price: $860.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium for Macrophages & Microglia Cells: Specialized freezing medium with higher FBS concentration, more suitable for Macrophages & Microglia Cells. | Size: 50 ml | CAT.#: 043-50 | Price: $85.00 | |
| RMcg Culture Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 100 ml | CAT.#: R619K-100 | Price: $85.00 |