Instructions RMbN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Midbrain Neurons (RMbN) are derived from midbrains of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions, RMbN arborize and form complex neurite network in one week. RMbN Stain positive for β III-Tubulin. Neurons are available in multiple formats, including cryopreserved neurons, freshly isolated primary neurons in suspension, or pre-plated cultured neurons.
The midbrain is a small part of the brain yet plays important functions in visual and auditory systems, motor control, alertness, sleep/wake, and temperature regulation. It contains the substantia nigra, a darkly pigmented region with high number of dopaminergic neurons, which is closely associated with motivation and habituation. Atrophies and diseases at midbrain seen in conditions such as Parkinson’s disease, familial amyotrophic lateral sclerosis, and progressive supranuclear palsy affect balancing, gait and muscle control.  Studying midbrain neurons in those disease conditions will allow a better understanding of the disease mechanisms, physiopathologies, and advancement in drug development and therapies. Cultures of midbrain neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.
(Click to Enlarge) Cryopreserved Rat Midbrain Neurons (RMbN) revived and cultured for 6 days (top), stained (bottom) on day 7 for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat midbrain 
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial1M RMbN in Serum-Free Frzng Med (042-50)
Fresh CultureContact CAI if interested in freshly isolated RMbN (suspension or plated)
KitCryovial frzn RMbN, Neuron Ctng Soln I (027-05), Plating (R817P-10) & Cultr Med (R817-100)
DoublingsN/A Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

 

 

Instructions RMbN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RMbN: Frozen RMb (1x10^6) Size: 1 Cryovial CAT.#: R8810N-10 Price: $378.00
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Cryopreserved RMbN : 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R8810NK-10 Price: $475.00
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Freshly isolated RMbN: Contact CAI for details Size: 1 Tube CAT.#: R8811NF-10 Price: $0.00
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Pre-plated freshly isolated RMbN: Contact CAI for details Size: 96 Well CAT.#: R8811NF-96W Price: $0.00
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Pre-plated freshly isolated RMbN: Contact CAI for details Size: 6 Well CAT.#: R8811NF-6W Price: $0.00
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Pre-plated freshly isolated RMbN: Contact CAI for details Size: 24 Well CAT.#: R8811NF-24W Price: $0.00
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Related Products

Product Size CAT.# Price Quantity
Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $31.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.00
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $31.00
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Rat Neuron Culture Medium: For the culture and maintenance of neurons. Size: 100 ml CAT.#: R817-100 Price: $63.00
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 100 ml CAT.#: R817K-100 Price: $72.00
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 10 ml CAT.#: R817P-10 Price: $20.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 20 ml CAT.#: R817P-20 Price: $39.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 40 ml CAT.#: R817P-40 Price: $62.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $404.00
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $54.00
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Rat Neuron Culture Medium: For the culture and maintenance of neurons Size: 150 ml CAT.#: R817-150 Price: $92.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now