Instructions RMbN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Midbrain Neurons (RMbN) are derived from midbrains of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions, RMbN arborize and form complex neurite network in one week. RMbN Stain positive for β III-Tubulin.
The midbrain is a small part of the brain yet plays important functions in visual and auditory systems, motor control, alertness, sleep/wake, and temperature regulation. It contains the substantia nigra, a darkly pigmented region with high number of dopaminergic neurons, which is closely associated with motivation and habituation. Atrophies and diseases at midbrain seen in conditions such as Parkinson’s disease, familial amyotrophic lateral sclerosis, and progressive supranuclear palsy affect balancing, gait and muscle control. Studying midbrain neurons in those disease conditions will allow a better understanding of the disease mechanisms, physiopathologies, and advancement in drug development and therapies. Cultures of midbrain neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.
(Click to Enlarge) Cryopreserved Rat Midbrain Neurons (RMbN) revived and cultured for 6 days (top), stained (bottom) on day 7 for β III-Tubulin (green), GFAP (red), and DAPI (blue).
Details
| Tissue | Normal healthy rat midbrain |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for β-III Tubulin |
| Bioassay | Plate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med |
| Cryovial | 1M RMbN in Serum-Free Frzng Med (042-50) |
| Kit | Cryovial frzn RMbN, Neuron Ctng Soln I (027-05), Plating (R817P-10) & Cultr Med (R817-100) |
| Doublings | N/A Neurons don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Midbrain Neurons Total Kit: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: R8810NK-10 | Price: $523.00 | |
| Cryopreserved Rat Midbrain Neurons (RMbN): Frozen RMb (1x10^6) | Size: 1 Cryovial | CAT.#: R8810N-10 | Price: $420.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Neuron Culture Medium: For the culture and maintenance of neurons. (For rat dorsal root gangion neurons, use Cat# R823K-100.) | Size: 100 ml | CAT.#: R817-100 | Price: $72.00 | |
| Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately. (For rat dorsal root gangion neurons, use Cat# R823K-100.) | Size: Yields 100 ml | CAT.#: R817K-100 | Price: $83.00 | |
| Rat Neuron Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 100 ml | CAT.#: R816-100 | Price: $40.00 | |
| Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium | Size: 3 ml | CAT.#: R817-GS | Price: $57.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 5 ml | CAT.#: 027-05 | Price: $18.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 10 ml | CAT.#: 027-10 | Price: $36.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 10 ml | CAT.#: R817P-10 | Price: $23.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 20 ml | CAT.#: R817P-20 | Price: $45.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 40 ml | CAT.#: R817P-40 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $465.00 | |
| Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $62.00 |
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