Instructions RMbN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
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Description

Rat Midbrain Neurons (RMbN) are derived from midbrains of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions, RMbN arborize and form complex neurite network in one week. RMbN Stain positive for β III-Tubulin. 
The midbrain is a small part of the brain yet plays important functions in visual and auditory systems, motor control, alertness, sleep/wake, and temperature regulation. It contains the substantia nigra, a darkly pigmented region with high number of dopaminergic neurons, which is closely associated with motivation and habituation. Atrophies and diseases at midbrain seen in conditions such as Parkinson’s disease, familial amyotrophic lateral sclerosis, and progressive supranuclear palsy affect balancing, gait and muscle control.  Studying midbrain neurons in those disease conditions will allow a better understanding of the disease mechanisms, physiopathologies, and advancement in drug development and therapies. Cultures of midbrain neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.
(Click to Enlarge) Cryopreserved Rat Midbrain Neurons (RMbN) revived and cultured for 6 days (top), stained (bottom) on day 7 for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat midbrain 
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial1M RMbN in Serum-Free Frzng Med (042-50)
  
KitCryovial frzn RMbN, Neuron Ctng Soln I (027-05), Plating (R817P-10) & Cultr Med (R817-100)
DoublingsN/A Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

 

 

Instructions RMbN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RMbN : 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R8810NK-10 Price: $467.00
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Cryopreserved RMbN: Frozen RMb (1x10^6) Size: 1 Cryovial CAT.#: R8810N-10 Price: $378.00
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Related Products

Product Size CAT.# Price Quantity
Rat Neuron Culture Medium: For the culture and maintenance of neurons. Size: 100 ml CAT.#: R817-100 Price: $63.00
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 100 ml CAT.#: R817K-100 Price: $72.00
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $31.00
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $31.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 10 ml CAT.#: R817P-10 Price: $20.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 20 ml CAT.#: R817P-20 Price: $39.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 40 ml CAT.#: R817P-40 Price: $62.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $404.00
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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