Instructions RNSC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description

Rat Neural Stem Cells (RNSC) are multipotent, and can be induced to differentiate into neurons, astrocytes and oligodendrocytes that make up the central nervous system (CNS). The ability of neural stem cells to retain multi-lineage potential and proliferate extensively in vitro provides new avenues for the treatment of neural degenerative diseases and injuries. Isolated from fetal or adult rat brains, RNSC are maintained in an undifferentiated, proliferative state by culturing them as free floating neurospheres in serum-free medium optimized with growth factors.
Image: The RNSC depicted here have formed neurospheres (top), and are stained for β-tubulin III (green) and GFAP (red) (bottom)
RNSC are cryopreserved at first passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation. Differentiated RNSCs are positive for β-tubulin III, GFAP and the oligodendrocyte marker O4 when cultured in Rat Neural Differentiation Medium. When cultured in Rat Neural Stem Cell Growth Medium, RNSC form the neurospheres that when transplanted into intact brain, survive, expand and differentiate into mature neurons, astrocytes and oligodendrocytes in 3 weeks.

(Click to Enlarge) Rat Neural Stem Cells (RNSC) (A) Stained for β-tubulin III (green) GFAP (red) and DAPI (B), β-tubulin III (blue) and GFAP (red), and β-tubulin III (green), GFAP (red) and DAPI (D)

Details

Tissue
Normal healthy rat brain cerebral cortex
QC
No bacteria, yeast, fungi, mycoplasma
Character
β-tubulin III, GFAP & O4 in Differentiation Med
Bioassay
Form neurosphere in RNSC Growth Med
Cryovial
2,000,000 RNSC (1st passage) cryopreserved in Freezing Med (040-50)
Kit
Cryovial frozen RNSC (RS820-20f), Growth Medium (R813-250), Neural Stem Cell Dissociation Solution (076-20), 10cm non-TC dish x2
Cultured
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 2
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RNSC

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Products

Product Size CAT.# Price Quantity
Cryopreserved RNSC, Fetal: 2x10^6 Cells (Fetal) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: RS820K-20f Price: $768.00
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Cryopreserved RNSC, Fetal: Frozen RNSC (2x10^6) Size: 1 Cryovial CAT.#: RS820-20f Price: $608.00
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Proliferating RNSC, Fetal: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: RS821-25f Price: $608.00
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Proliferating RNSC, Fetal: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: RS821-75f Price: $813.00
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Proliferating RNSC, Fetal: Actively growing, dividing cells, in medium Size: 6 Well CAT.#: RS821-6Wf Price: $813.00
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Proliferating RNSC, Fetal: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: RS821-96Wf Price: $942.00
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Related Products

Product Size CAT.# Price Quantity
RNSC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 250 ml CAT.#: R812-250 Price: $40.00
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RNSC Differentiation Medium: General RNSC Differentiation Medium Size: 250 ml CAT.#: R813D-250 Price: $128.00
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RNSC Differentiation Medium, Astrocyte Lineage: Differentiates RNSC to Astrocytes Size: 100 ml CAT.#: R813D-100A Price: $62.00
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RNSC Differentiation Medium, Neuron Lineage: Differentiates RNSC to Neurons Size: 100 ml CAT.#: R813D-100N Price: $74.00
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RNSC Differentiation Medium, Oligodendrocyte Lineage: Differentiates RNSC to Oligodendrocytes Size: 100 ml CAT.#: R813D-100O Price: $62.00
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RNSC Growth Medium: All-in-one ready-to-use Size: 250 ml CAT.#: R813-250 Price: $118.00
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RNSC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: yields 250 ml CAT.#: R813K-250 Price: $128.00
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RNSC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 10 ml CAT.#: R813-GS Price: $88.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now