Instructions RPF
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Perineurial Fibroblasts (RPF) from Cell Applications, Inc. are of mesenchymal origin and form the perineurium, one of the three connective tissue compartments of the peripheral nerve. The perineurium wraps around nerve fascicles to form a metabolically active diffusion barrier. It provides protection to the nerve bundles, maintains the homeostasis of the endoneurium, modulates external stretching forces that exerts to the nerve, and regulates endoneurial pressures.
RPF and other cells of mesenchymal origin express the cytoplasmic protein Vimentin, a Type III intermediate filament that forms part of the cytoskeleton. By helping to maintain cell shape, cytoplasmic integrity and cytoskeletal interactions, Vimentin functions in cell adhesion, migration and signaling.
Perineurial fibroblasts also express fibronectin, a multifunctional extracellular matrix glycoprotein. Like vimentin, fibronectin plays major roles in cell morphology, cytoskeletal organization, adhesion, growth and migration. Fibronectin, which binds other ECM molecules, also functions in cell differentiation, embryonic development, hemostasis, phagocytosis, wound healing and repair.
(Click to Enlarge) Rat Perineurial Fibroblasts (RPF) revived (top) and stained for Vimentin (green, bottom left), Fibronectin (green, bottom right), and DAPI (blue).
Details
| Tissue | Normal healthy rat peripheral nerves |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for Fibronectin |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 RPF (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen RPF (R852-05n), Growth Medium (R827-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 1st psg (flasks or plates) |
| Doublings | At least 12 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Perineurial Fibroblasts Total Kit: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R852K-05n | Price: $563.00 | |
| Cryopreserved Rat Perineurial Fibroblasts (RPF), neonatal: Frozen RPF (5x10^5) | Size: 1 Cryovial | CAT.#: R852-05n | Price: $390.00 | |
| Proliferating Rat Perineurial Fibroblasts (RPF), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R853-25n | Price: $390.00 | |
| Proliferating Rat Perineurial Fibroblasts (RPF), neonatal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R853-24Wn | Price: $580.00 | |
| Proliferating Rat Perineurial Fibroblasts (RPF), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R853-75n | Price: $580.00 | |
| Proliferating Rat Perineurial Fibroblasts (RPF), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R853-96Wn | Price: $700.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| RPF Growth Medium: All-in-one ready-to-use, for Rat Perineurial Fibroblasts. | Size: 500 ml | CAT.#: R827-500 | Price: $120.00 | |
| RPF Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R827K-500 | Price: $127.00 | |
| RPF Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R826-500 | Price: $74.00 | |
| RPF Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 25 ml | CAT.#: R827-GS | Price: $73.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Vimentin Antibody: Mouse Vimentin Antibody | Size: 100 ul | CAT.#: CP10244 | Price: $333.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $465.00 | |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $62.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Rat Fibronectin ELISA Kit: Rat Fibronectin ELISA Kit | Size: 96 Wells | CAT.#: CL0350 | Price: $500.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |