Instructions RSpN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Spinal Cord Neurons (RSpN) are derived from spinal cords of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions, RSpN arborize and form complex neurite network in one week. RSpN Stain positive for β III-Tubulin. 
The spinal cord is consisted of tubular bundles of nervous tissues and support cells. Extending from the medulla oblongata of the brainstem, the spinal cord transmits neural signals between the brain and the rest of the body, and plays a key role in somatosensory and motor functions. Spinal cord injuries, diseases and degeneration, such as Friedreich's ataxia, Amyotrophic lateral sclerosis, and spinal muscular atrophies, cause weakening or loss of sensory and motor functions.  Studies using spinal cord neurons will allow a better understanding of the disease mechanisms, physiopathologies, and advancement in drug development and therapies. Cultures of spinal cord neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.
(Click to Enlarge) Cryopreserved Rat Spinal Cord Neurons (RSpN) revived and cultured for 6 days (top), stained (bottom) on day 7 for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat spinal cord 
QCNo bacteria, yeast, fungi, mycoplasma, virus 
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, Arborize to form neurite network in Culture Med
Cryovial2 or 4M RSpN in Ser-Fr Frzng Med (042-50)
  
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

 

 

Instructions RSpN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RSpN: Frozen RSpN (2x10^6) Size: 1 Cryovial CAT.#: R8814N-20 Price: $707.00
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Cryopreserved RSpN: Frozen RSpN (3x10^6) Size: 1 Cryovial CAT.#: R8814N-40 Price: $1,281.00
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Related Products

Product Size CAT.# Price Quantity
Rat Neuron Culture Medium: For the culture and maintenance of neurons. Size: 100 ml CAT.#: R817-100 Price: $63.00
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 100 ml CAT.#: R817K-100 Price: $72.00
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $31.00
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $31.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 10 ml CAT.#: R817P-10 Price: $20.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 20 ml CAT.#: R817P-20 Price: $39.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 40 ml CAT.#: R817P-40 Price: $62.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $404.00
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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