Instructions RStN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Striatal Neurons (RStN) are derived from striatum of normal embryonic rat (gestation day 18) by standardized methods. When cultured under the recommended conditions, RStN arborize and form complex neurite network in one week. RStN Stain positive for β III-Tubulin. Neurons are available in multiple formats, including cryopreserved neurons, freshly isolated primary neurons in suspension, or pre-plated cultured neurons.
The striatum is a subcortical part of the forebrain. It receives and processes input signals from different parts of the cerebral cortex. With its robust interactions among cholinergic, dopaminergic, and GABAergic neurotransmitter systems, striatum plays a key role in voluntary movement, reward-based habit learning, addiction, procedural memory formation, and cognitive dysfunctions in Parkinson’s disease. Studies using striatal neurons will allow a better understanding of the striatal-related functions and diseases. Cultures of striatal neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.
(Click to Enlarge) Cryopreserved Rat Striatal Neurons (RStN) revived and cultured for 8 days (top), stained (bottom) for β III-Tubulin (green), GFAP (red), and DAPI (blue).
Details
| Tissue | Normal healthy rat striatum |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for β-III Tubulin |
| Bioassay | Plate on Poly-D-Lysine coated surface, Arborize to form neurite network in Culture Med |
| Cryovial | 1M RStN in Serum-free Frzng Med (042-50) |
| Fresh Culture | Contact CAI if interested in freshly isolated RStN (suspension or plated) |
| Kit | Cryovial frzn RStN (R8812N-10), Neuron Ctng Soln I (027-05), Plating (R817P-10) & Cultr Med (R817-100) |
| Doublings | N/A - Neurons don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved RStN: Frozen RStN (1x10^6) | Size: 1 Cryovial | CAT.#: R8812N-10 | Price: $302.38 | |
| Cryopreserved RStN Total Kit: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: R8812NK-10 | Price: $400.80 | |
| Freshly isolated RStN: Contact CAI for details | Size: 1 Tube | CAT.#: R8813NF-10 | Price: $0.00 | |
| Pre-plated freshly isolated RStN: Contact CAI for details | Size: 6 Well | CAT.#: R8813NF-6W | Price: $0.00 | |
| Pre-plated freshly isolated RStN: Contact CAI for details | Size: 96 Well | CAT.#: R8813NF-96W | Price: $0.00 | |
| Pre-plated freshly isolated RStN: Contact CAI for details | Size: 24 Well | CAT.#: R8813NF-24W | Price: $0.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 10 ml | CAT.#: 027-10 | Price: $30.75 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 5 ml | CAT.#: 027-05 | Price: $16.14 | |
| Rat Neuron Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 100 ml | CAT.#: R816-100 | Price: $31.21 | |
| Rat Neuron Culture Medium : For the culture and maintenance of neurons | Size: 150 ml | CAT.#: R817-150 | Price: $92.25 | |
| Rat Neuron Culture Medium : For the culture and maintenance of neurons. | Size: 100 ml | CAT.#: R817-100 | Price: $63.50 | |
| Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 100 ml | CAT.#: R817K-100 | Price: $72.11 | |
| Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium | Size: 3 ml | CAT.#: R817-GS | Price: $57.04 | |
| Rat Neuron Plating Medium: For initial plating of neurons | Size: 20 ml | CAT.#: R817P-20 | Price: $38.95 | |
| Rat Neuron Plating Medium: For initial plating of neurons | Size: 40 ml | CAT.#: R817P-40 | Price: $61.50 | |
| Rat Neuron Plating Medium: For initial plating of neurons | Size: 10 ml | CAT.#: R817P-10 | Price: $20.45 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Colorimetric Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $138.84 | |
| Cyto-X Colorimetric Cell Counting Reagent: 100 tests | Size: Sample | CAT.#: 028-S | Price: $35.88 | |
| Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $403.59 | |
| Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $53.76 | |
| Rat Neuron Culture Medium : For the culture and maintenance of neurons | Size: 150 ml | CAT.#: R817-150 | Price: $92.25 |