Description
Total RNA prepared from normal human tissues using the modified guanidium thiocyanate method. RNA is DNAse treated to remove contaminating genomic DNA and undergoes stringent quality control tests. Purified RNA is ready for use in convenient 50 & 250 µg sizes for various downstream applications, including microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.
See also Cell RNA
(Click to Enlarge) Tissue RNA. Human Fetal Brain (lines 2, 3) and Human Adult Lung (lines 4, 5) Total RNA. RNA was electrophoresed in 1% agarose gel to show the distinct 28S and 18S ribosomal RNA bands (lines 2, 4). RT-PCR was performed with HSP60 primers to produce 2.2 kb product (lines 3, 5).
Details
Stringent quality control tests confirm RNA integrity and stability:
- Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37C incubation
- No visible Genomic DNA band of 23 kb detected
- No genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primers
- A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers
- A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis
- Primers were designed to detect mRNA for genes of interest to confirm cell phenotype
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal | Size: 50 ug | CAT.#: 1F01-50 | Price: $198.00 | |
| Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal | Size: 250 ug | CAT.#: 1F01-250 | Price: $595.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 50 ug | CAT.#: 1H30-50 | Price: $158.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 250 ug | CAT.#: 1H30-250 | Price: $595.00 | |
| Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 50 ug | CAT.#: 1H50-50 | Price: $158.00 | |
| Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 250 ug | CAT.#: 1H50-250 | Price: $595.00 | |
| Human Liver RNA, Fetal : Total RNA prepared from human liver tissue, fetal | Size: 50 ug | CAT.#: 1F21-50 | Price: $198.00 | |
| Human Liver RNA, Fetal : Total RNA prepared from human liver tissue, fetal | Size: 250 ug | CAT.#: 1F21-250 | Price: $595.00 | |
| Human Liver RNA: Total RNA prepared from human liver tissue | Size: 50 ug | CAT.#: 1H21-50 | Price: $158.00 | |
| Human Liver RNA: Total RNA prepared from human liver tissue | Size: 250 ug | CAT.#: 1H21-250 | Price: $595.00 | |
| Human Lung RNA, Fetal : Total RNA prepared from human lung tissue | Size: 50 ug | CAT.#: 1F40-50 | Price: $158.00 | |
| Human Lung RNA, Fetal : Total RNA prepared from human lung tissue | Size: 250 ug | CAT.#: 1F40-250 | Price: $595.00 | |
| Human Lung RNA: Total RNA prepared from human lung tissue | Size: 50 ug | CAT.#: 1H40-50 | Price: $158.00 | |
| Human Lung RNA: Total RNA prepared from human lung tissue | Size: 250 ug | CAT.#: 1H40-250 | Price: $595.00 | |
| Human Pancreas RNA: Total RNA prepared from human pancreas tissue | Size: 50 ug | CAT.#: 1H23-50 | Price: $158.00 | |
| Human Pancreas RNA: Total RNA prepared from human pancreas tissue | Size: 250 ug | CAT.#: 1H23-250 | Price: $595.00 | |
| Human Placenta RNA: Total RNA prepared from human placental tissue | Size: 50 ug | CAT.#: 1H54-50 | Price: $158.00 | |
| Human Placenta RNA: Total RNA prepared from human placental tissue | Size: 250 ug | CAT.#: 1H54-250 | Price: $595.00 | |
| Human Prostate RNA: Total RNA prepared from human prostate tissue | Size: 50 ug | CAT.#: 1H52-50 | Price: $158.00 | |
| Human Prostate RNA: Total RNA prepared from human prostate tissue | Size: 250 ug | CAT.#: 1H52-250 | Price: $595.00 | |
| Human Skeletal Muscle RNA, Fetal: Total RNA prepared from human skeletal muscle tissue, fetal | Size: 50 ug | CAT.#: 1F60-50 | Price: $198.00 | |
| Human Skeletal Muscle RNA, Fetal: Total RNA prepared from human skeletal muscle tissue, fetal | Size: 250 ug | CAT.#: 1F60-250 | Price: $740.00 | |
| Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue | Size: 50 ug | CAT.#: 1H60-50 | Price: $158.00 | |
| Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue | Size: 250 ug | CAT.#: 1H60-250 | Price: $595.00 | |
| Human Small Intestine RNA: Total RNA prepared from human small intestine tissue | Size: 50 ug | CAT.#: 1H24-50 | Price: $158.00 | |
| Human Small Intestine RNA: Total RNA prepared from human small intestine tissue | Size: 250 ug | CAT.#: 1H24-250 | Price: $595.00 | |
| Human Spleen RNA: Total RNA prepared from human spleen tissue | Size: 50 ug | CAT.#: 1H70-50 | Price: $158.00 | |
| Human Spleen RNA: Total RNA prepared from human spleen tissue | Size: 250 ug | CAT.#: 1H70-250 | Price: $595.00 | |
| Human Stomach RNA: Total RNA prepared from human stomach tissue | Size: 50 ug | CAT.#: 1H20-50 | Price: $158.00 | |
| Human Stomach RNA: Total RNA prepared from human stomach tissue | Size: 250 ug | CAT.#: 1H20-250 | Price: $595.00 | |
| Human Testes RNA: Total RNA prepared from human testes tissue | Size: 50 ug | CAT.#: 1H51-50 | Price: $158.00 | |
| Human Testes RNA: Total RNA prepared from human testes tissue | Size: 250 ug | CAT.#: 1H51-250 | Price: $595.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Custom RNA Services: Total RNA from Human Tissues | Size: 1 Project | CAT.#: CSTSVC-RHT | Price: $0.00 |