VEGF-120: Mouse Vascular Endothelial Growth Factor-120

Product Sheet RP2052

Description

BACKGROUND Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilatation via nitric oxide-dependent pathways. Mouse VEGF-A has several variants, one being VEGF-120. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%.

Recombinant mouse VEGF-120 is an alternative spliced version of Mouse VEGF-165, consisting of a non-glycosylated homodimer with a molecular weight of 28.2 kDa.

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat. No.:
RP2052
 
Alternative Name:
VEGF-A, VPF, glioma-derived endothelial cell mitogen
 
Source:
E. coli
 
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
 
Formulation:
Recombinant mouse VEGF-120 is lyophilized with no additives.
 
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
 
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
 
Biological Activity:
The activity is determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) and is typically 1-5 ng/mL.
 
AA Sequence:
APTTEGEQKS HEVIKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCAGCC NDEALECVPT SESNITMQIM RIKPHQSQHI GEMSFLQHSR CECRPKKDRT KPEKCDKRPRR
 

Products

Product Size CAT.# Price Quantity
Mouse Vascular Endothelial Growth Factor-120 (VEGF-120): Mouse Vascular Endothelial Growth Factor-120 Size: 10 ug CAT.#: RP2052-10 Price: $194.00
-+
Mouse Vascular Endothelial Growth Factor-120 (VEGF-120): Mouse Vascular Endothelial Growth Factor-120 Size: 100 ug CAT.#: RP2052-100 Price: $484.00
-+
Mouse Vascular Endothelial Growth Factor-120 (VEGF-120): Mouse Vascular Endothelial Growth Factor-120 Size: 1000 ug CAT.#: RP2052-1000 Price: $4,090.00
-+

Resources/Documents

Product Sheet RP2052