Instructions Gastrointestinal EpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules

Description

Crohn's Disease (CD) is an inflammatory bowel disease (IBD) that causes chronic inflammation of the gastrointestinal tract. While Crohn’s Disease most commonly affects the ileum and colon, the disease can affect any part of the GI tract from the mouth to the anus. An estimated 3 million Americans are affected by IBD. While genetics (positive family history for IBD is the greatest known risk factor) and immune response play a role in the disease, the cause of the disorder is unknown. And while standard treatment (anti-inflammatory steroids and immunosuppressants) can slow progression of disease, there is no known cure. 

To better understand the disease and how to treat it, Cell Applications offers researchers access to an unprecedented biobank of Epithelial Cells from Crohn’s Disease cases. The Cell Applications biobank includes a diverse cohort of disease age, location, and behavior. Sample locations include colon (Cn), ileum (Il), Rectum (Re). CD cells are categorized as untreated (naive) and Treated Crohn's Disease. Generally, naive cells are from Crohn's Disease cases exhibiting non-stricturing, non-penetrating behavior (B1), while stricturing (B2) and penetrating (B3) CD patients have sought treatment.

For your convenience, the Epithelial Cell Total Kit includes all the reagents you need for gastrointestinal epithelial cell culture. Along with a cryovial of 500,000 diseased epithelial cells, the Total Kit includes optimized cell culture medium, thawing medium, and coating solution. Cells can be cultured up to 7 days. Cells cannot be passaged and should be plated directly into the experimental system. 

Learn more about:

Crohn's Disease Gastrointestinal Epithelial Cells, Naive (HCnEpC-CD-N): phase contrast 100x, immunostained for ZO-1 (red), Villin (green) and Na-K-ATPase (green), 200x.

Details

TissueHuman colon (Cn) or ileum (Il) of untreated Crohn's Disease.
QCNo bacteria, yeast, fungi, mycoplasma, virus.
BioassayAttach, spread, in Culture Medium.
Cryovial500,000 HIlEpC-CD-N HCnpC-CD-N in Freezing Medium.
KitCryovial frozen HIlEpC-CD-N (732Il-CD-N-05a) or HCnEpC-CD-N (732Cn-CD-N-05a), with Culture Med (716DC-50), Coating Solution (025-05), Thawing Solution (716T-20).
CulturedShipped in flasks or plates in medium.
DoublingsCells can be cultured up to 7 days. Cells do not divide, and cannot be passaged. 
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions Gastrointestinal EpC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HCnEpC-CD-N, adult : Frozen HCnEpC-CD-N (5x10^5) from left colon, Ileitis, 55 yr old male with untreated Crohn's disease. Size: 1 Cryovial CAT.#: 732Cn-CD-N-05a-0130023 Price: $1,150.00
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Cryopreserved HIlEpC-CD-N, adult: Frozen HIlEpC-CD-N (5x10^5) from ileum, left-sided colitis, 19 yr old male with untreated Crohn's disease. Size: 1 Cryovial CAT.#: 732Il-CD-N-05a-0120021 Price: $1,150.00
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Cryopreserved HCnEpC-CD-N, adult : Frozen HCnEpC-CD-N (5x10^5)  from left colon, Ileocolitis, 29 yr old male with untreated Crohn's disease. Size: 1 Cryovial CAT.#: 732Cn-CD-N-05a-0260058 Price: $1,150.00
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Cryopreserved HIlEpC-CD-N, adult: Frozen HIlEpC-CD-N (5x10^5) from ileum, ileitis, 71yr old female with untreated Crohn's disease. Size: 1 Cryovial CAT.#: 732Il-CD-N-05a-0300083 Price: $1,150.00
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Related Products

Product Size CAT.# Price Quantity
GI Epithelial Cell Defined Culture Medium: All-in-one medium, includes antibiotics. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716DC-50 Price: $55.00
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GI Epithelial Cell Thawing Solution: Thawing solution for GI Epithelial Cells Size: 20 ml CAT.#: 716T-20 Price: $26.00
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GI Epithelial Cell Coating Solution: Coating solution for GI Epithelial Cells Size: 5 ml CAT.#: 025-05 Price: $16.00
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Extended Family Products

Product Size CAT.# Price Quantity
TEER Assay GI Epithelial Cell Medium : All-in-one ready-to-use optimized media for studying GI epithelial barrier function. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716TA-50 Price: $65.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

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5 Important Cell Culture Rules

Format: PDF

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